PXD006643

PXD006643 is an original dataset announced via ProteomeXchange.

Title	Integrative genomic and proteomic analysis of response of Lactobacillus casei str. Zhang to glucose limitation
Description	Nutrient starvation is an important survival challenge for bacteria during industrial production of functional foods. Lactobacilli are increasingly being used as probiotics in functional foods. As next-generation sequencing technology has greatly advanced, we performed integrative proteomic and genomic analysis to investigate the response of Lactobacillus casei Zhang to a glucose-restricted environment. L. casei Zhang strains were permitted to evolve in glucose-limited or normal medium from a common ancestor over a 3-year period, and they were sampled after 1000, 2000, 3000, 4000, 5000, 6000, 7000, and 8000 generations and subjected to proteomic and genomic analyses. Genomic resequencing data revealed different point mutations and other mutational events in each generation of L. casei Zhang under glucose limitation stress. The proteins expressed differentially under glucose limitation were found to be significantly related to fructose and mannose metabolism, carbohydrate metabolic processes, lyase activity, and amino acid-transporting ATPase activity. The integrative proteomic and genomic analysis revealed that the mutations protected L. casei Zhang against glucose starvation by regulating other cellular carbohydrate, fatty acid, and amino acid catabolism; phosphoenolpyruvate system pathway activation; glycogen synthesis; ATP consumption; pyruvate metabolism; and general stress response protein expression. The results help reveal the mechanisms of adapting to glucose starvation and provide new strategies for enhancing the industrial utility of L. casei Zhang.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:38:02.173.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jie Yu
SpeciesList	scientific name: Lactobacillus casei str. Zhang; NCBI TaxID: 498216;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2017-06-01 02:15:34	ID requested
1	2018-02-08 03:13:34	announced
⏵ 2	2024-10-22 04:38:02	announced	2024-10-22: Updated project metadata.

10.1021/acs.jproteome.7b00886;

Yu J, Hui W, Cao C, Pan L, Zhang H, Zhang W, Integrative Genomic and Proteomic Analysis of the Response of Lactobacillus casei Zhang to Glucose Restriction. J Proteome Res, 17(3):1290-1299(2018) [pubmed]

curator keyword: Biological

submitter keyword: Lactobacillus casei str. Zhang ;lace of glucose

Jie Yu
contact affiliation	Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University.
contact email	yunbiopride@foxmail.com
lab head
Jie Yu
contact affiliation	Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University.
contact email	43439408@qq.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD006643
     1. Label: PRIDE project
     2. Name: Integrative genomic and proteomic analysis of response of Lactobacillus casei str. Zhang to glucose limitation