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PXD006639

DataSet Summary

  • HostingRepository: PRIDE
  • AnnounceDate: 2017-11-29
  • AnnouncementXML: Submission_2017-12-11_03:57:27.xml
  • DigitalObjectIdentifier:
  • ReviewLevel: Peer-reviewed dataset
  • DatasetOrigin: Original data
  • RepositorySupport: Unsupported dataset by repository
  • PrimarySubmitter: Marc Presler
  • Title: Proteomics of phosphorylation and protein dynamics during fertilization and meiotic exit in the Xenopus egg
  • Description: Fertilization triggers release from meiotic arrest and initiates events that prepare for the ensuing developmental program. Protein degradation and phosphorylation are known to regulate protein activity during this process. However, the full extent of protein loss and phospho-regulation is still unknown. We examined absolute protein and phospho-site dynamics after fertilization by mass spectrometry-based proteomics. To do this, we developed a new approach for calculating the stoichiometry of phospho-sites from multiplexed proteomics compatible with dynamic, stable and multi-site phosphorylation. Overall, the data suggest that degradation is limited to a few low abundance proteins. However, this degradation in part promotes extensive dephosphorylation that occurs over a wide range of abundances during meiotic exit. We also show that eggs release a large amount of protein into the medium just after fertilization, most likely related to the blocks to polyspermy. Concomitantly, there is a substantial increase in phosphorylation likely tied to calcium activated kinases. We identify putative degradation targets as well as new components of the block to polyspermy. The analytical approaches demonstrated here are broadly applicable to studies of dynamic biological systems.
  • SpeciesList: scientific name: Xenopus laevis (African clawed frog); NCBI TaxID: 8355;
  • ModificationList: TMT6plex-126 reporter+balance reagent acylated residue; phosphorylated residue
  • Instrument: LTQ Orbitrap Elite

Dataset History

VersionDatetimeStatusChangeLog Entry
02017-06-01 00:45:13ID requested
12017-11-29 03:02:58announced
22017-12-11 03:57:28announcedUpdated publication reference for PubMed record(s): 29183978.

Publication List

  1. Presler M, Van Itallie E, Klein AM, Kunz R, Coughlin ML, Peshkin L, Gygi SP, Wühr M, Kirschner MW, egg. Proc Natl Acad Sci U S A, 114(50):E10838-E10847(2017) [pubmed]

Keyword List

  1. curator keyword: Biological
  2. submitter keyword: Xenopus, egg, fertilization,

Contact List

    Marc W. Kirschner
    • contact affiliation: Department of Systems Biology, Harvard Medical School, Boston, MA 02115, USA
    • contact email: marc@hms.harvard.edu
    • lab head:
    Marc Presler
    • contact affiliation: Graduate Student
    • contact email: marcpresler@fas.harvard.edu
    • dataset submitter:

Full Dataset Link List

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Repository Record List
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