PXD006635

PXD006635 is an original dataset announced via ProteomeXchange.

Title	Plant UDP-N-acetylglucosamine (UDP-GlcNAc) transporter (UGNT1) glycopeptide analysis
Description	Glycosylation reactions require activated glycosyl donors in form of nucleotide sugars to drive processes such as post-translational protein modifications, glycolipid and polysaccharide biosynthesis. Most of these reactions occur in the Golgi requiring cytosolic-derived nucleotide sugars, which are actively transferred into the Golgi lumen by nucleotide sugar transporters. Here we present the identification of the plant UDP-N-acetylglucosamine (UDP-GlcNAc) transporter (UGNT1) indispensable for the delivery of a substrate for maturation of N-glycans and glycosyl inositol phosphorylceramides (GIPCs). Profiles of N-glycopeptides revealed that UGNT1 loss-of-function mutants are devoid of complex and hybrid N-glycans. Instead, most of the glycol-N-peptide population contained high mannose structures, representing the structure prior to the addition of the first GlcNAc in the Golgi. Our findings emphasize that the reference plant Arabidopsis contains a single UDP-GlcNAc transporter responsible for the maturation of complex N-glycans in the Golgi lumen.
HostingRepository	PRIDE
AnnounceDate	2018-09-24
AnnouncementXML	Submission_2018-09-24_04:12:30.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kris Ford
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2017-05-31 00:17:29	ID requested
⏵ 1	2018-09-24 04:12:31	announced

Ebert B, Rautengarten C, McFarlane HE, Rupasinghe T, Zeng W, Ford K, Scheller HV, Bacic A, Roessner U, Persson S, Heazlewood JL, A Golgi UDP-GlcNAc transporter delivers substrates for N-linked glycans and sphingolipids. Nat Plants, 4(10):792-801(2018) [pubmed]

curator keyword: Biological

submitter keyword: glycopeptide, nucleotide sugar transporters, UDP-N-acetylglucosamine

Joshua L. Heazlewood
contact affiliation	1. School of Biosciences, The University of Melbourne, Melbourne, VIC 3010, Australia 2. Joint BioEnergy Institute and Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA
contact email	jheazlewood@unimelb.edu.au
lab head
Kris Ford
contact affiliation	School of Botany
contact email	kferg@unimelb.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/09/PXD006635

PRIDE project URI

• PRIDE
  1. PXD006635
     1. Label: PRIDE project
     2. Name: Plant UDP-N-acetylglucosamine (UDP-GlcNAc) transporter (UGNT1) glycopeptide analysis