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PXD006635

PXD006635 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePlant UDP-N-acetylglucosamine (UDP-GlcNAc) transporter (UGNT1) glycopeptide analysis
DescriptionGlycosylation reactions require activated glycosyl donors in form of nucleotide sugars to drive processes such as post-translational protein modifications, glycolipid and polysaccharide biosynthesis. Most of these reactions occur in the Golgi requiring cytosolic-derived nucleotide sugars, which are actively transferred into the Golgi lumen by nucleotide sugar transporters. Here we present the identification of the plant UDP-N-acetylglucosamine (UDP-GlcNAc) transporter (UGNT1) indispensable for the delivery of a substrate for maturation of N-glycans and glycosyl inositol phosphorylceramides (GIPCs). Profiles of N-glycopeptides revealed that UGNT1 loss-of-function mutants are devoid of complex and hybrid N-glycans. Instead, most of the glycol-N-peptide population contained high mannose structures, representing the structure prior to the addition of the first GlcNAc in the Golgi. Our findings emphasize that the reference plant Arabidopsis contains a single UDP-GlcNAc transporter responsible for the maturation of complex N-glycans in the Golgi lumen.
HostingRepositoryPRIDE
AnnounceDate2018-09-24
AnnouncementXMLSubmission_2018-09-24_04:12:30.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterKris Ford
SpeciesList scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationListiodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02017-05-31 00:17:29ID requested
12018-09-24 04:12:31announced
Publication List
Ebert B, Rautengarten C, McFarlane HE, Rupasinghe T, Zeng W, Ford K, Scheller HV, Bacic A, Roessner U, Persson S, Heazlewood JL, A Golgi UDP-GlcNAc transporter delivers substrates for N-linked glycans and sphingolipids. Nat Plants, 4(10):792-801(2018) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: glycopeptide, nucleotide sugar transporters, UDP-N-acetylglucosamine
Contact List
Joshua L. Heazlewood
contact affiliation1. School of Biosciences, The University of Melbourne, Melbourne, VIC 3010, Australia 2. Joint BioEnergy Institute and Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA
contact emailjheazlewood@unimelb.edu.au
lab head
Kris Ford
contact affiliationSchool of Botany
contact emailkferg@unimelb.edu.au
dataset submitter
Full Dataset Link List
Dataset FTP location
PRIDE project URI
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