PXD006632

PXD006632 is an original dataset announced via ProteomeXchange.

Title	Evolutionary proteomics uncovers ciliary signaling components
Description	Cilia are organelles specialized for movement and signaling. To infer when during animal evolution signaling pathways became associated with cilia, we characterized the proteomes of cilia from three organisms: sea urchins, sea anemones and choanoflagellates. From these ciliomes, we identified 437 high confidence ciliary candidate proteins conserved in mammals, including known regulators of Hh, GPCR and TRP channel signaling. The phylogenetic profiles of their ciliary association indicate that the Hh and GPCR pathways were linked to cilia before the origin of bilateria and TRP channels before the origin of animals. We demonstrated that some of the candidates not previously implicated in ciliary biology localized to cilia and further investigated ENKUR, a TRP channel-interacting protein that we identified in the cilia of all three organisms. In animals, ENKUR is expressed by cells with motile cilia, ENKUR localizes to cilia in diverse organisms and, in both Xenopus laevis and mice, ENKUR is required for patterning the left/right axis. Moreover, mutation of ENKUR causes situs inversus in humans. Thus, proteomic profiling of cilia from diverse eukaryotes defines a conserved ciliary proteome, reveals ancient connections to Hh, GPCR and TRP channel signaling, and uncovers a novel ciliary protein that controls vertebrate development and human disease.
HostingRepository	PRIDE
AnnounceDate	2019-12-16
AnnouncementXML	Submission_2019-12-16_03:58:54.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jeffrey Johnson
SpeciesList	scientific name: Nematostella vectensis; NCBI TaxID: 45351;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2017-05-30 23:52:15	ID requested
1	2019-12-16 02:52:04	announced
⏵ 2	2019-12-16 03:58:55	announced	2019-12-16: Updated project metadata.

Sigg MA, Menchen T, Lee C, Johnson J, Jungnickel MK, Choksi SP, Garcia G, Busengdal H, Dougherty GW, Pennekamp P, Werner C, Rentzsch F, Florman HM, Krogan N, Wallingford JB, Omran H, Reiter JF, Evolutionary Proteomics Uncovers Ancient Associations of Cilia with Signaling Pathways. Dev Cell, 43(6):744-762.e11(2017) [pubmed]

curator keyword: Biological

submitter keyword: sea urchin, Strongylocentrotus purpuratus, sea anemone, Nematostella vectensis, Choanoflagellate, Salpingoeca rosetta, cilia, signaling, evolution

Jeremy Reiter
contact affiliation	University of California San Francisco
contact email	Jeremy.Reiter@ucsf.edu
lab head
Jeffrey Johnson
contact affiliation	UCSF
contact email	jeffrey.johnson@ucsf.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/12/PXD006632

PRIDE project URI

• PRIDE
  1. PXD006632
     1. Label: PRIDE project
     2. Name: Evolutionary proteomics uncovers ciliary signaling components