PXD006626

PXD006626 is an original dataset announced via ProteomeXchange.

Title	Capturing protein communities by structural proteomics in a thermophilic eukaryote
Description	The arrangement of proteins into complexes is a key organizational principle for many cellular functions. Although the topology of many complexes has been systematically analyzed in isolation, their molecular sociology in situ remains elusive. Here, we show that crude cellular extracts of a eukaryotic thermophile, Chaetomium thermophilum, retain basic principles of cellular organization. Using a structural proteomics approach, we simultaneously characterized the abundance, interactions and structure of a third of the C. thermophilum proteome within these extracts. We identified 27 distinct protein communities that include 108 interconnected complexes, which dynamically associate with each other and functionally benefit from being in close proximity in the cell. Furthermore, we investigated the structure of fatty acid synthase within these extracts by cryoEM and this revealed multiple, flexible states of the enzyme in adaptation to its association with other complexes, thus exemplifying the need for in situ studies. As the components of the captured protein communities are known – at both the protein and complex level – this study constitutes another step forward towards a molecular understanding ofsubcellular organization.
HostingRepository	PRIDE
AnnounceDate	2017-07-26
AnnouncementXML	Submission_2017-07-26_05:03:44.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Francis O'Reilly
SpeciesList	scientific name: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719); NCBI TaxID: 759272;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2017-05-30 06:44:25	ID requested
⏵ 1	2017-07-26 05:03:45	announced

Dataset with its publication pending

curator keyword: Biological

submitter keyword: CLMS, SEC, Chaetomium thermophilum

Juri Rappsilber
contact affiliation	1. Department of Bioanalytics, Institute of Biotechnology, Technische Universität Berlin, 13355 Berlin, Germany, 2. Wellcome Trust Centre for Cell Biology, School of Biological Sciences, University of Edinburgh, Edinburgh EH9 3JR, United Kingdom
contact email	juri.rappsilber@tu-berlin.de
lab head
Francis O'Reilly
contact affiliation	TU Berlin
contact email	oreilly@tu-berlin.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD006626
     1. Label: PRIDE project
     2. Name: Capturing protein communities by structural proteomics in a thermophilic eukaryote