PXD006615

PXD006615 is an original dataset announced via ProteomeXchange.

Title	Comparison of targeted mass spectrometry techniques with an immunoassay: a case study for HSP90 alpha
Description	We developed 2D-LC-MS/MS-based SRM and PRM assays to measure HSP90 alpha in serum and compared the results to a commercially available immunoassay (ELISA). Serum samples were trypsin-digested and fractionated by SCX chromatography prior to SRM and PRM measurements. PRM data obtained by high-resolution mass spectrometry correlated better with ELISA measurements than SRM data measured on a triple quadrupole mass spectrometer. While all three methods (SRM, PRM, ELISA) were able to quantify HSP90 alpha in serum at the ng/mL level, the use of PRM on a high-resolution mass spectrometer reduced variation. To rule out that the observed differences in SRM and PRM are due to different mass spectrometry systems, the SCX fractions were measured on the same high-resolution instrument (Orbitrap) in the ion trap mode (IT-PRM); such measurements showed that intense co-eluting signals were present in the SRM method, but these interfering peaks were eliminated in the high-resolution PRM mode. Thus, this report shows that it is possible to measure ng/mL levels of HSP90 alpha in a reproducible, selective and sensitive way using PRM. This opens up the possibility to quantify low levels of multiple proteins in complex samples based on a fractionation strategy on tryptic peptides followed by PRM.
HostingRepository	PRIDE
AnnounceDate	2018-07-05
AnnouncementXML	Submission_2018-07-05_06:53:05.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD006615
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Coskun Guzel
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Oxidation; Carbamidomethyl
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2017-05-29 04:54:45	ID requested
⏵ 1	2018-07-05 06:53:06	announced

G, ü, zel C, Govorukhina NI, Stingl C, Dekker LJM, Boichenko A, van der Zee AGJ, Bischoff RPH, Luider TM, . Proteomics Clin Appl, 12(1):(2018) [pubmed]

curator keyword: Technical

submitter keyword: Serum, SCX, HSP90, SRM, PRM, DDA, shotgun, ELISA

Theo Luider
contact affiliation	Head of Laboratory of Neuro-Oncology Clinical & Cancer Proteomics Erasmus MC the Netherlands
contact email	t.luider@erasmusmc.nl
lab head
Coskun Guzel
contact affiliation	Erasmus MC
contact email	c.guzel@erasmusmc.nl
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD006615
     1. Label: PRIDE project
     2. Name: Comparison of targeted mass spectrometry techniques with an immunoassay: a case study for HSP90 alpha