PXD006604

PXD006604 is an original dataset announced via ProteomeXchange.

Title	Cotton fiber LC-MS/MS
Description	Upland cotton (Gossypium hirsutum L.) is one of the world’s most important fiber crops, accounting for more than 90% of all cotton production. While their wild progenitors have relatively short and coarse, often tan-colored fibers, modern cotton cultivars possess longer, finer, stronger, and whiter fiber. In this study, the wild and cultivated cottons (YU-3 and TM-1) selected show significant differences on fibers at 10 day post-anthesis (DPA), 20 DPA and mature stages at the physiological level. In order to explore the effects of domestication, reveal molecular mechanisms underlying these phenotypic differences and better inform our efforts to further enhance cotton fiber quality, an iTRAQ-facilitated proteomic methods were performed on developing fibers. There were 6990 proteins identified, among them 336 were defined as differentially expressed proteins (DEPs) between fibers of wild versus domesticated cotton. The down- or up-regulated proteins in wild cotton were involved in Phenylpropanoid biosynthesis, Zeatin biosynthesis, Fatty acid elongation and other processes. Association analysis between transcroptome and proteome showed positive correlations between transcripts and proteins at both 10 DPA and 20 DPA. The difference of proteomics had been verified at the mRNA level by qPCR, also at physiological and biochemical level by POD activity determination and ZA content estimation. This work corroborate the major pathways involved in cotton fiber development and demonstrate that POD activity and zeatin content have a great potential related to fiber elongation and thickening.
HostingRepository	PRIDE
AnnounceDate	2017-08-28
AnnouncementXML	Submission_2017-08-28_02:31:29.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yuan Qin
SpeciesList	scientific name: Gossypium hirsutum (Upland cotton) (Gossypium mexicanum); NCBI TaxID: 3635;
ModificationList	iTRAQ8plex-116 reporter+balance reagent acylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-05-26 04:45:53	ID requested
⏵ 1	2017-08-28 02:31:31	announced

Qin Y, Wei H, Sun H, Hao P, Wang H, Su J, Yu S, Proteomic Analysis of Differences in Fiber Development between Wild and Cultivated Gossypium hirsutum L. J Proteome Res, 16(8):2811-2824(2017) [pubmed]

curator keyword: Biological

submitter keyword: Gossypium hirsutun, fiber development, domestication, iTRAQ

Shuxun Yu
contact affiliation	Institute of Cotton Research, Chinese Academy of Agricultural Sciences, No.38 Huanghe Road, Anyang, Henan 455000, China
contact email	Ysx195311@163.com
lab head
Yuan Qin
contact affiliation	State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, No.38 Huanghe Road, Anyang, Henan 455000, China. Phone: +86 18272203876
contact email	qinyuan564@qq.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD006604
     1. Label: PRIDE project
     2. Name: Cotton fiber LC-MS/MS