PXD006598

PXD006598 is an original dataset announced via ProteomeXchange.

Title	Dynamic Acclimation to High Light in Arabidopsis thaliana Involves Widespread Reengineering of the Leaf Proteome
Description	Typically, when fully developed leaves of Arabidopsis thaliana are exposed to an increase in light intensity, they are able to increase their photosynthetic capacity in a process known as dynamic acclimation. Fully developed leaves of Arabidopsis thaliana were exposed to a fourfold increase in light intensity for 7 days to induce high light acclimation. This treatment was subjected to wild-type and a non-acclimating mutant lacking the gpt2 gene. The proteomic responses of the leaves were investigated using label-free mass spectrometry. A large reorganisation of the proteome was shown, with increases in the abundance of proteins of photosynthesis and carbon metabolism. Subtle differences were seen between the WT and gpt2 mutant: in the mutant, an increased stress response was seen, and some differences in the responses of metabolism. Proteomic responses generally correlated with physiological responses.
HostingRepository	PRIDE
AnnounceDate	2017-07-20
AnnouncementXML	Submission_2017-08-07_05:21:33.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD006598
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Matthew Miller
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	Oxidation; Carbamidomethyl
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2017-05-26 04:40:02	ID requested
1	2017-07-20 04:36:25	announced
⏵ 2	2017-08-07 05:21:34	announced	Updated publication reference for PubMed record(s): 28775726.

Miller MAE, O'Cualain R, Selley J, Knight D, Karim MF, Hubbard SJ, Johnson GN, Involves Widespread Reengineering of the Leaf Proteome. Front Plant Sci, 8():1239(2017) [pubmed]

curator keyword: Biological

submitter keyword: Arabidopsis, label free, proteomics, high light acclimation, dynamic acclimation

Giles N Johnson
contact affiliation	School of Earth and Environmental Sciences, University of Manchester
contact email	giles.johnson@manchester.ac.uk
lab head
Matthew Miller
contact affiliation	Agrimetrics
contact email	matt.miller@agrimetrics.co.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD006598
     1. Label: PRIDE project
     2. Name: Dynamic Acclimation to High Light in Arabidopsis thaliana Involves Widespread Reengineering of the Leaf Proteome