PXD006596

PXD006596 is an original dataset announced via ProteomeXchange.

Title	Translational repression of the Drosophila nanos mRNA involves the RNA helicase Belle and RNA coating by Me31B and Trailer hitch
Description	The maternal mRNA nanos is required for anterior-posterior patterning in the early Drosophila melanogaster development. The nanos-mRNA is translationally repressed and deadenylated during the first two hours of embryogenesis. A stem-loop structure in the 3'-UTR of this RNA (SRE - Smaug recogniiton element) is bound by the Smaug protein which recruits a protein complex to the RNA to sequester its translation and intiate transcript degradation via the ccr4/not-complex. To analyse the composition of this repressor complex, biotinylated RNAs carrying two SRE stem-loops were used for an affinity purification of bound proteins. As a negative control an RNA was used that contained two point mutations in the SRE loop, that abolish binding of Smaug. Seven subunits were identified as components of the repressor complex. The main deadenylase in Drosophila, the ccr4/not-complex was also significantly enriched.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:38:10.014.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michael Götze
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2017-05-26 04:38:50	ID requested
1	2017-07-18 00:53:03	announced
2	2017-09-12 03:00:40	announced	Updated publication reference for PubMed record(s): 28701521.
⏵ 3	2024-10-22 04:38:18	announced	2024-10-22: Updated project metadata.

G, ö, tze M, Dufourt J, Ihling C, Rammelt C, Pierson S, Sambrani N, Temme C, Sinz A, Simonelig M, Wahle E, mRNA involves the RNA helicase Belle and RNA coating by Me31B and Trailer hitch. RNA, 23(10):1552-1568(2017) [pubmed]

10.1261/rna.062208.117;

curator keyword: Biological

submitter keyword: Cup, Ccr4-not-complex, deadenylation DEAD-box helicase, Trailer hitch, nanos, Me31B, translational repression,Smaug

Elmar Wahle
contact affiliation	Institute for Biochemistry and Biotechnology, Martin Luther University Halle-Wittenberg
contact email	ewahle@biochemtech.uni-halle.de
lab head
Michael Götze
contact affiliation	University Halle-Witenberg
contact email	michael.goetze@biochemtech.uni-halle.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD006596
     1. Label: PRIDE project
     2. Name: Translational repression of the Drosophila nanos mRNA involves the RNA helicase Belle and RNA coating by Me31B and Trailer hitch