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DataSet Summary

  • HostingRepository: PRIDE
  • AnnounceDate: 2017-08-11
  • AnnouncementXML: Submission_2017-08-11_03:15:12.xml
  • DigitalObjectIdentifier:
  • ReviewLevel: Peer-reviewed dataset
  • DatasetOrigin: Original data
  • RepositorySupport: Unsupported dataset by repository
  • PrimarySubmitter: Martina Samiotaki
  • Title: Human Angiogenin Nuclear Interactions
  • Description: Human Angiogenin (hAng) is a member of the ribonuclease A superfamily and a potent inducer of neovascularization. Herein, protein interactions of hAng in the nucleus and cytoplasm of the human umbilical vein cell line EA.hy926, have been investigated by mass spectroscopy. The first gel-free analysis of hAng immunoprecipitates from the cytoplasmic and nuclear extracts from EA.hy926 cancer human cell line, revealed many statistically significant potential hAng-interacting proteins. The majority of the proteins identified by the mass spectrometry analysis are parts of multiprotein complexes, and some of them are common in nucleus and cytoplasm, including spliceosome, proteasome, and molecular chaperone TRiC/CCT. These complexes are involved in crucial biological pathways, implicating thus the key role of hAng in many vital cellular processes.
  • SpeciesList: scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
  • ModificationList: deaminated residue; monohydroxylated residue; iodoacetamide derivatized residue
  • Instrument: LTQ Orbitrap

Dataset History

VersionDatetimeStatusChangeLog Entry
02017-05-24 07:35:23ID requested
12017-08-11 03:15:13announced

Publication List

  1. Chatzileontiadou DSM, Samiotaki M, Alexopoulou AN, Cotsiki M, Panayotou G, Stamatiadi M, Balatsos NAA, Leonidas DD, Kontou M, Proteomic Analysis of Human Angiogenin Interactions Reveals Cytoplasmic PCNA as a Putative Binding Partner. J Proteome Res, 16(10):3606-3622(2017) [pubmed]

Keyword List

  1. curator keyword: Biological
  2. submitter keyword: Angiogenin, Immunoprecipitation, LC-MS/MS

Contact List

    Maria Kontou
    • contact affiliation: Department of Biochemistry and Biotechnology, University of Thessaly, Biopolis, 41500 Larissa, Greece
    • contact email: mkontou@bio.uth.gr
    • lab head:
    Martina Samiotaki
    • contact affiliation: Protein Analysis Laboratory B.S.R.C. "Alexander Fleming", Alexander Fleming Street 34 16672, Vari, Greece
    • contact email: samiotaki@fleming.gr
    • dataset submitter:

Full Dataset Link List

  1. Dataset FTP location
  2. PRIDE project URI
Repository Record List
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