PXD006577

PXD006577 is an original dataset announced via ProteomeXchange.

Title	Proteomics analysis of streptomyces sp. MBT76 demonstrates the production of qinichelins, new mixed catecholate-hydroxamate siderophores
Description	Actinomycete genomes contain a plethora of orphan gene clusters encoding unknown secondary metabolites, and representing a huge unexploited pool of chemical diversity. The explosive increase in genome sequencing and the massive advance of bioinformatic tools have revolutionized the rationale for natural product discovery from actinomycetes. In this context, we applied a genome mining approach to discover a group of unique catecholate-hydroxamate siderophores termed as qinichelins from Streptomyces sp. MBT76. Quantitative proteomics statistically correlated a gene cluster of interest (qch) to its unknown chemotype (qinichelin), after which structural elucidation of isolated qinichelin was assisted by bioinformatics analysis and verified by MS2 and NMR experiments. Strikingly, intertwined functional crosstalk among four separately located gene clusters was implicated in the biosynthesis of qinichelins.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:38:08.381.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jacob Gubbens
SpeciesList	scientific name: Streptomyces sp. MBT76; NCBI TaxID: 1488406;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2017-05-24 04:39:51	ID requested
1	2018-10-24 12:48:39	announced
⏵ 2	2024-10-22 04:38:09	announced	2024-10-22: Updated project metadata.

Gubbens J, Wu C, Zhu H, Filippov DV, Florea BI, Rigali S, Overkleeft HS, van Wezel GP, Intertwined Precursor Supply during Biosynthesis of the Catecholate-Hydroxamate Siderophores Qinichelins in Streptomyces sp. MBT76. ACS Chem Biol, 12(11):2756-2766(2017) [pubmed]

10.1021/acschembio.7b00597;

submitter keyword: streptomyces, dimethyl labeling, siderophore, natural products,LC-MSMS

Prof.dr. G.P. van Wezel
contact affiliation	Molecular Biotechnology, Institute of Biology, Leiden University, Sylviusweg 72, 2333 BE, The Netherlands
contact email	g.wezel@biology.leidenuniv.nl
lab head
Jacob Gubbens
contact affiliation	Leiden University
contact email	j.gubbens@biology.leidenuniv.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD006577

PRIDE project URI

• PRIDE
  1. PXD006577
     1. Label: PRIDE project
     2. Name: Proteomics analysis of streptomyces sp. MBT76 demonstrates the production of qinichelins, new mixed catecholate-hydroxamate siderophores