PXD006575

PXD006575 is an original dataset announced via ProteomeXchange.

Title	Specific labeling of stem cell activity in human colorectal organoids using an ASCL2-responsive minigene
Description	Organoid technology provides the possibility to culture human colon tissue and patient-derived colorectal cancers (CRC) while maintaining all functional and phenotypic characteristics. Labeling of human colon stem cells (CoSCs), especially in normal and benign tumor organoids, is challenging and therefore limits usability of multi-patient organoid libraries for CoSC research. Here, we developed STAR (STem cell Ascl2 Reporter), a minimal enhancer/promoter element that reports transcriptional activity of ASCL2, a master regulator of LGR5+ CoSC fate. Among others via lentiviral infection, STAR minigene labels stem cells in normal as well as in multiple engineered and patient-derived CRC organoids of different stage and genetic make-up. STAR revealed that stem cell driven differentiation hierarchies and the capacity of cell fate plasticity (de-differentiation) are present at all stages of human CRC development. The flexible and user-friendly nature of STAR applications in combination with organoid technology will facilitate basic research on human adult stem cell biology.
HostingRepository	PRIDE
AnnounceDate	2018-01-29
AnnouncementXML	Submission_2019-02-12_08:24:53.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rik Lindeboom
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	dimethylated residue; iodoacetamide derivatized amino-terminal residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2017-05-23 06:17:23	ID requested
1	2018-01-29 08:03:14	announced
⏵ 2	2019-02-12 08:24:55	announced	Updated publication reference for PubMed record(s): 29425513.

Oost KC, van Voorthuijsen L, Fumagalli A, Lindeboom RGH, Sprangers J, Omerzu M, Rodriguez-Colman MJ, Heinz MC, Verlaan-Klink I, Maurice MM, Burgering BMT, van Rheenen J, Vermeulen M, Snippert HJG, Specific Labeling of Stem Cell Activity in Human Colorectal Organoids Using an ASCL2-Responsive Minigene. Cell Rep, 22(6):1600-1614(2018) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: Organoids, intestine, colorectal cancer, stem cells, LGR5, ASCL2

Hugo Snippert
contact affiliation	Molecular Cancer Research, Center for Molecular Medicine, University Medical Center Utrecht, Utrecht, The Netherlands
contact email	H.J.G.Snippert@umcutrecht.nl
lab head
Rik Lindeboom
contact affiliation	Radboud Institute for Molecular Life Sciences
contact email	r.lindeboom@science.ru.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/01/PXD006575

PRIDE project URI

• PRIDE
  1. PXD006575
     1. Label: PRIDE project
     2. Name: Specific labeling of stem cell activity in human colorectal organoids using an ASCL2-responsive minigene