PXD006568

PXD006568 is an original dataset announced via ProteomeXchange.

Title	Comparative proteomics of reduced protein phosphatase inhibitor-2 expression in the loss of function mutant Arabidopsis
Description	Protein phosphatase inhibitor-2 (PPI-2) is a conserved eukaryotic effector protein that inhibits type one protein phosphatases (TOPP). A transfer-DNA knockdown of AtPPI-2 resulted in stunted growth in both vegetative and reproductive phases of Arabidopsis development. At the cellular level, AtPPI-2 knockdown had 35 to 40% smaller cells in developing roots and leaves. This developmental phenotype was rescued by transgenic expression of the AtPPI-2 cDNA behind a constitutive promoter. Comparative proteomics of developing leaf proteins of wild type (WT) and AtPPI-2 mutant revealed reduced levels of proteins associated with chloroplast development, ribosome biogenesis, transport, and cell cycle regulation processes. Down-regulation of several ribosomal proteins, a DEAD box RNA helicase family protein (AtRH3), Clp protease (ClpP3) and proteins associated with cell division suggests a bottleneck in chloroplast ribosomal biogenesis and cell cycle regulation in AtPPI-2 mutant plants. In contrast, eight out of nine Arabidopsis TOPP isoforms were up-regulated at the transcript level in AtPPI-2 leaves compared to WT. A protein-protein interaction network revealed that more than 75% of the differentially expressed proteins have at least secondary and/or tertiary connections with AtPPI-2. Collectively, these data reveal a potential basis for the growth defects of AtPPI-2 and support the presumed role of AtPPI-2 as a master regulator for TOPPs, which regulate diverse growth and developmental processes.
HostingRepository	PRIDE
AnnounceDate	2017-12-14
AnnouncementXML	Submission_2017-12-14_00:07:39.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nagib Ahsan
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap; LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2017-05-23 03:29:38	ID requested
⏵ 1	2017-12-14 00:07:40	announced

Ahsan N, Chen M, Salvato F, Wilson RS, Shyama Prasad Rao R, Thelen JJ, Comparative proteomic analysis provides insight into the biological role of protein phosphatase inhibitor-2 from Arabidopsis. J Proteomics, 165():51-60(2017) [pubmed]

curator keyword: Biological

submitter keyword: type-1 phosphatase, phosphatase inhibitor, comparative proteomics, ribosome biogenesis, protein-protein interaction, PPI-2, Arabidopsis

Jay J. Thelen
contact affiliation	Professor, University of Missouri-Columbia, Department of Biochemistry, 271G Life Sciences Center, Columbia, MO 65211, USA
contact email	thelenj@missouri.edu
lab head
Nagib Ahsan
contact affiliation	Brown University
contact email	nagib_ahsan@brown.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD006568
     1. Label: PRIDE project
     2. Name: Comparative proteomics of reduced protein phosphatase inhibitor-2 expression in the loss of function mutant Arabidopsis