PXD006564

PXD006564 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	DIAPH1 interaction partner identification using BioID.
Description	293T cells were transfected with p TRIPZ BirA tagged DIAPH1. Two days post transfection, cells were treated with puromycin to select transfectants. The entire BirA-DIAPH1 cassette is under the control of Tet inducible promoter. Induction of DIAPH1 was achieved by treating cells with Doxycycline (10ug/ml) for 24hrs followed by 50uM of Biotin treatment, overnight, to induce biotinylation of interacting partners. Cells treated with Biotin alone (no Dox) were used as a control for IP. Cytoplasmic extracts were prepared and subjected to immunoprecipitation using Streptavidin magnetic beads. Beads were washed with RIPA buffer and subjected to Mass spectrometry based protein identification to find interacting partners of DIAPH1.
HostingRepository	jPOST
AnnounceDate	2017-05-23
AnnouncementXML	Submission_2022-09-18_03:48:47.914.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gopinath M
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; L-methionine sulfoxide
Instrument	instrument

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2017-05-22 20:03:35	ID requested
1	2017-05-22 20:03:39	announced
2	2018-09-19 00:13:22	announced	Updated CV, FTP location and Modification list.
3	2021-02-04 16:41:35	announced	2021-02-04: Updated FTP location.
⏵ 4	2022-09-18 03:48:48	announced	2022-09-18: Updated FTP location.

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: DIAPH1-BioID

submitter keyword: DIAPH1-BioID

Contact List

Dr. Prabha Sampath
lab head
Gopinath M
contact affiliation	Agency for Science Technology and Research
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000273/

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000273/