PXD006559

PXD006559 is an original dataset announced via ProteomeXchange.

Title	Proteogenomics of ex vivo-activated platelets.
Description	Platelets are major players in the process of intravascular thrombus formation. Current therapeutic strategies still fail to prevent thrombotic coronary events in a substantial number of patients, indicating that the complex mechanisms modulating platelet response during activation are not fully elucidated. The evidence that platelets are capable of de novo protein synthesis has raised the issue of whether and how these resident mRNAs are regulated in circulating platelets. Among the several mechanisms potentially involved, mRNA splicing may be relevant. Purified platelet-rich plasmas from healthy volunteers were collected and in vitro activated with collagen or Thrombin Receptor Activating Peptide (TRAP). Transcriptome profiling by RNA-Seq and in silico intron representation analysis were applied to search for the presence of pre-mRNA molecules and splicing events affected by platelet activation. HiRIEF LC-MS allowed platelet proteome characterization at deep coverage to investigate a possible correlation between splicing events and protein levels. By comparing computational and wet-lab analyses it was possible to identify a set of transcripts influenced at both intron and protein level.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:37:59.194.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mattias Vesterlund
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-05-22 05:40:10	ID requested
1	2018-01-03 00:33:28	announced
2	2018-01-16 01:53:58	announced	Updated publication reference for PubMed record(s): 29323256.
⏵ 3	2024-10-22 04:38:05	announced	2024-10-22: Updated project metadata.

Nassa G, Giurato G, Cimmino G, Rizzo F, Ravo M, Salvati A, Nyman TA, Zhu Y, Vesterlund M, Lehti, ö J, Golino P, Weisz A, Tarallo R, Splicing of platelet resident pre-mRNAs upon activation by physiological stimuli results in functionally relevant proteome modifications. Sci Rep, 8(1):498(2018) [pubmed]

10.1038/s41598-017-18985-5;

curator keyword: Biological, Biomedical

submitter keyword: human, proteome,platelet activation, cytoplasmic pre-mRNA splicing

Janne Lehtiö
contact affiliation	Cancer proteomics, Oncology-Pathology department, Karolinska Institutet
contact email	janne.lehtio@ki.se
lab head
Mattias Vesterlund
contact affiliation	Karolinska Institutet
contact email	mattias.vesterlund@ki.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/01/PXD006559

PRIDE project URI

• PRIDE
  1. PXD006559
     1. Label: PRIDE project
     2. Name: Proteogenomics of ex vivo-activated platelets.