PXD006556

PXD006556 is an original dataset announced via ProteomeXchange.

Title	Targeted proteomics of signal pathways in BV2 microglial cell activation
Description	Microglia become activated by disturbances in the homeostasis of their local microenvironment. While there are varying degrees of microglia activation, a pro-inflammatory reactive state induced by exposure to stimuli like LPS and IFN-γ. In this study, using label-free PRM approach which is allowing us to measure the hyper-multiplex quantitative value of each protein with high-resolution mass spectrometry, we have quantified over 450 peptides at single run corresponding with the proteins belong to the pathways and directly or indirectly interacted proteins based on STRING database.Moreover, we performed longitudinal quantification of secreted proteins during the detrimental activation of microglia which releases neurotoxic molecules mediated neuronal cell loss in the brain region.
HostingRepository	PRIDE
AnnounceDate	2017-08-09
AnnouncementXML	Submission_2017-08-09_01:01:57.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jongmin Woo
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; iodoacetamide derivatized amino-terminal residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-05-22 05:31:55	ID requested
⏵ 1	2017-08-09 01:01:58	announced

Woo J, Han D, Wang JI, Park J, Kim H, Kim Y, Quantitative Proteomics Reveals Temporal Proteomic Changes in Signaling Pathways during BV2 Mouse Microglial Cell Activation. J Proteome Res, 16(9):3419-3432(2017) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: BV2 activation, quantitative analysis, label-free PRM, Targeted protoemics

Youngsoo Kim
contact affiliation	Departments of Biomedical Sciences, Seoul National University, Seoul, Korea
contact email	biolab@snu.ac.kr
lab head
Jongmin Woo
contact affiliation	Seoul National University
contact email	jongminw@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/08/PXD006556

PRIDE project URI

• PRIDE
  1. PXD006556
     1. Label: PRIDE project
     2. Name: Targeted proteomics of signal pathways in BV2 microglial cell activation