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PXD006550

PXD006550 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteome and peptidome of human follicular fluid
DescriptionHuman follicular fluid (hFF) is a natural environment of oocyte maturation, and some components of hFF could be used to judge oocyte capability for fertilization and further development. In our pilot small scale study three samples from four donors (12 samples in total) were analyzed to determine which hFF proteins/peptides could be used to differentiate individual oocytes and which are patient specific. Ultrafiltration was used to fractionate hFF to High Molecular Weight (HMW) – proteome (>10kDa) and Low Molecular Weight (LMW) – peptidome (<10 kDa) fractions. HMW and LMW compositions were analyzed using LC-MS in SWATH data acquisition and processing methodology. In total we were able to identify 158 proteins, form which 59 were never reported before as hFF components. 55 (45 Never reported before) proteins were found by analyzing LMW fraction, 67 (14 never reported before) were found by analyzing HMW fraction, and 36 were identified in both fractions of hFF. We were able to perform quantitative analysis for 72 proteins from HMW fraction of hFF. We found that concentrations of 18 proteins varied substantially among hFF samples from single donors and those proteins are promising targets to identify biomarkers useful in oocyte quality assessment.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:38:07.332.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAleksandra Bogucka
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentTripleTOF 5600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02017-05-19 06:49:07ID requested
12017-07-04 06:51:22announced
22024-10-22 04:38:14announced2024-10-22: Updated project metadata.
Publication List
10.1021/acs.jproteome.7b00366;
Lewandowska AE, Macur K, Czaplewska P, Liss J, Ł, ukaszuk K, O, ł, dziej S, Qualitative and Quantitative Analysis of Proteome and Peptidome of Human Follicular Fluid Using Multiple Samples from Single Donor with LC-MS and SWATH Methodology. J Proteome Res, 16(8):3053-3067(2017) [pubmed]
Keyword List
curator keyword: Biological, Biomedical
submitter keyword: Human, Follicular Fluid, SWATH, LC-MS/MS
Contact List
Stanislaw Oldziej
contact affiliationLaboratory of Biopolymer Structure, Intercollegiate Faculty of Biotechnology UG-MUG, University of Gdansk, Gdansk, Poland
contact emailstanislaw.oldziej@biotech.ug.edu.pl
lab head
Aleksandra Bogucka
contact affiliationJustus Liebig University Giessen
contact emailaleksandra.lewandowska@biotech.ug.edu.pl
dataset submitter
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Dataset FTP location
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PRIDE project URI
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