PXD006550

PXD006550 is an original dataset announced via ProteomeXchange.

Title	Proteome and peptidome of human follicular fluid
Description	Human follicular fluid (hFF) is a natural environment of oocyte maturation, and some components of hFF could be used to judge oocyte capability for fertilization and further development. In our pilot small scale study three samples from four donors (12 samples in total) were analyzed to determine which hFF proteins/peptides could be used to differentiate individual oocytes and which are patient specific. Ultrafiltration was used to fractionate hFF to High Molecular Weight (HMW) – proteome (>10kDa) and Low Molecular Weight (LMW) – peptidome (<10 kDa) fractions. HMW and LMW compositions were analyzed using LC-MS in SWATH data acquisition and processing methodology. In total we were able to identify 158 proteins, form which 59 were never reported before as hFF components. 55 (45 Never reported before) proteins were found by analyzing LMW fraction, 67 (14 never reported before) were found by analyzing HMW fraction, and 36 were identified in both fractions of hFF. We were able to perform quantitative analysis for 72 proteins from HMW fraction of hFF. We found that concentrations of 18 proteins varied substantially among hFF samples from single donors and those proteins are promising targets to identify biomarkers useful in oocyte quality assessment.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:38:07.332.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Aleksandra Bogucka
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2017-05-19 06:49:07	ID requested
1	2017-07-04 06:51:22	announced
⏵ 2	2024-10-22 04:38:14	announced	2024-10-22: Updated project metadata.

10.1021/acs.jproteome.7b00366;

Lewandowska AE, Macur K, Czaplewska P, Liss J, Ł, ukaszuk K, O, ł, dziej S, Qualitative and Quantitative Analysis of Proteome and Peptidome of Human Follicular Fluid Using Multiple Samples from Single Donor with LC-MS and SWATH Methodology. J Proteome Res, 16(8):3053-3067(2017) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: Human, Follicular Fluid, SWATH, LC-MS/MS

Stanislaw Oldziej
contact affiliation	Laboratory of Biopolymer Structure, Intercollegiate Faculty of Biotechnology UG-MUG, University of Gdansk, Gdansk, Poland
contact email	stanislaw.oldziej@biotech.ug.edu.pl
lab head
Aleksandra Bogucka
contact affiliation	Justus Liebig University Giessen
contact email	aleksandra.lewandowska@biotech.ug.edu.pl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/07/PXD006550

PRIDE project URI

• PRIDE
  1. PXD006550
     1. Label: PRIDE project
     2. Name: Proteome and peptidome of human follicular fluid