PXD006550 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteome and peptidome of human follicular fluid |
Description | Human follicular fluid (hFF) is a natural environment of oocyte maturation, and some components of hFF could be used to judge oocyte capability for fertilization and further development. In our pilot small scale study three samples from four donors (12 samples in total) were analyzed to determine which hFF proteins/peptides could be used to differentiate individual oocytes and which are patient specific. Ultrafiltration was used to fractionate hFF to High Molecular Weight (HMW) – proteome (>10kDa) and Low Molecular Weight (LMW) – peptidome (<10 kDa) fractions. HMW and LMW compositions were analyzed using LC-MS in SWATH data acquisition and processing methodology. In total we were able to identify 158 proteins, form which 59 were never reported before as hFF components. 55 (45 Never reported before) proteins were found by analyzing LMW fraction, 67 (14 never reported before) were found by analyzing HMW fraction, and 36 were identified in both fractions of hFF. We were able to perform quantitative analysis for 72 proteins from HMW fraction of hFF. We found that concentrations of 18 proteins varied substantially among hFF samples from single donors and those proteins are promising targets to identify biomarkers useful in oocyte quality assessment. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:38:07.332.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Aleksandra Bogucka |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | TripleTOF 5600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2017-05-19 06:49:07 | ID requested | |
1 | 2017-07-04 06:51:22 | announced | |
⏵ 2 | 2024-10-22 04:38:14 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1021/acs.jproteome.7b00366; |
Lewandowska AE, Macur K, Czaplewska P, Liss J, Ł, ukaszuk K, O, ł, dziej S, Qualitative and Quantitative Analysis of Proteome and Peptidome of Human Follicular Fluid Using Multiple Samples from Single Donor with LC-MS and SWATH Methodology. J Proteome Res, 16(8):3053-3067(2017) [pubmed] |
Keyword List
curator keyword: Biological, Biomedical |
submitter keyword: Human, Follicular Fluid, SWATH, LC-MS/MS |
Contact List
Stanislaw Oldziej |
contact affiliation | Laboratory of Biopolymer Structure, Intercollegiate Faculty of Biotechnology UG-MUG, University of Gdansk, Gdansk, Poland |
contact email | stanislaw.oldziej@biotech.ug.edu.pl |
lab head | |
Aleksandra Bogucka |
contact affiliation | Justus Liebig University Giessen |
contact email | aleksandra.lewandowska@biotech.ug.edu.pl |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/07/PXD006550 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD006550
- Label: PRIDE project
- Name: Proteome and peptidome of human follicular fluid