PXD006545

PXD006545 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Sequential Peptide Immunopurification Methodology to Monitor the Crosstalk between SUMOylation and Ubiquitylation
Description	We describe a method that permits the identification of proteins that are modified by both SUMOylation and ubiquitylation to better understand the role of this crosstalk. The procedure requires 3 days when starting from cell pellets and can yield more than 8000 SUMO sites and 3500 ubiquitin sites from 16 mg of cell extract.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:37:55.815.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Francis McManus
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	ubiquitinylated lysine; phosphorylated residue; sumoylated lysine
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2017-05-19 00:27:57	ID requested
1	2018-03-27 02:52:53	announced
⏵ 2	2024-10-22 04:38:03	announced	2024-10-22: Updated project metadata.

Publication List

McManus FP, Lamoliatte F, Thibault P, Identification of cross talk between SUMOylation and ubiquitylation using a sequential peptide immunopurification approach. Nat Protoc, 12(11):2342-2358(2017) [pubmed]
10.1038/nprot.2017.105;

McManus FP, Lamoliatte F, Thibault P, Identification of cross talk between SUMOylation and ubiquitylation using a sequential peptide immunopurification approach. Nat Protoc, 12(11):2342-2358(2017) [pubmed]

10.1038/nprot.2017.105;

Keyword List

curator keyword: Biological
submitter keyword: SUMO, Ubiquitin, Shotgun proteomics

curator keyword: Biological

submitter keyword: SUMO, Ubiquitin, Shotgun proteomics

Contact List

Pierre Thibault
contact affiliation	University of Montréal, Department of Chemistry, Québec, Canada
contact email	francis.mcmanus@umontreal.ca
lab head
Francis McManus
contact affiliation	University of Montreal
contact email	francispmcmanus@gmail.com
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/03/PXD006545
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/03/PXD006545

PRIDE project URI

Repository Record List

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