PXD006544

PXD006544 is an original dataset announced via ProteomeXchange.

Title	Interactome of Ca2+-bound secretagogin in INS-1E cells
Description	By using His6-tagged recombinant human secretagogin in the presence of Ca2+ (100 µM), we precipitated putative interacting partners from INS-1E cells and identified their amino acid sequences by mass spectrometry. We detected canonical interacting proteins participating in vesicle-mediated transport, exocytosis and cytoskeletal organization. Besides, we captured proteins controlling protein folding and (de-)ubiquitination. Our data support that secretagogin modulates protein folding and degradation through protein-protein interactions and interacts with USP9X in β cells to control cell survival.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:37:52.814.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Edit Szodorai
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-05-18 07:32:14	ID requested
1	2017-06-21 01:10:49	announced
⏵ 2	2024-10-22 04:37:53	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

curator keyword: Biological, Biomedical

submitter keyword: INS-1E cell line,His6-tagged recombinant secretagogin, pull down, protein-protein interactions

Univ. Prof. Dr. Tibor Harkany
contact affiliation	Department of Molecular Neurosciences, Center for Brain Research, Medical University of Vienna, Vienna, Austria
contact email	tibor.harkany@meduniwien.ac.at
lab head
Edit Szodorai
contact affiliation	Dept. of Molecular Neurosciences, Center for Brain Research, Medical University of Vienna, Vienna, Austria
contact email	edit.szodorai@meduniwien.ac.at
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/06/PXD006544

PRIDE project URI

• PRIDE
  1. PXD006544
     1. Label: PRIDE project
     2. Name: Interactome of Ca2+-bound secretagogin in INS-1E cells