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PXD006543 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA map of protein-metabolite interactions reveals principles of chemical communication
DescriptionApplication of a novel mass spectrometry-based high-throughput workflow (LiP-SMap) and data resource based on limited proteolysis (LiP) of complex samples. Proteome-wide limited proteolysis sites were obtained in different conditions to infer ligand-induced and protein-protein interaction induced conformational changes. This information was used to identify unknown ligand binding proteins, small molecule-protein binding sites and the remodeling of protein complexes directly in cells.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterIlaria Piazza
SpeciesList scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion; Q Exactive; Q Exactive Plus
Dataset History
RevisionDatetimeStatusChangeLog Entry
02017-05-18 02:10:02ID requested
12018-01-04 02:22:59announced
22018-01-10 00:45:58announcedUpdated publication reference for PubMed record(s): 29307493.
Publication List
Piazza I, Kochanowski K, Cappelletti V, Fuhrer T, Noor E, Sauer U, Picotti P, A Map of Protein-Metabolite Interactions Reveals Principles of Chemical Communication. Cell, 172(1-2):358-372.e23(2018) [pubmed]
Keyword List
curator keyword: Technical, Biological
submitter keyword: DIA-MS, small molecules, E.coli, limited proteolysis, LiP-SMap
Contact List
Paola Picotti
contact affiliationInstitute of Molecular Systems Biology, ETH Zurich, Switzerland
contact emailpaola.picotti@imsb.biol.ethz.ch
lab head
Ilaria Piazza
contact affiliationETH Zurich
contact emaililaria.piazza@bc.biol.ethz.ch
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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