PXD006541

PXD006541 is an original dataset announced via ProteomeXchange.

Title	LC-MSMS of cell secretome for MAP1LC3B interactors
Description	Growing evidence implicates autophagy in cell secretion. Identifying the repertoire of proteins involved with autophagy dependent secretions is key for understanding the underlying mechanism. We use a proximity-dependent biotinylation proteomics strategy to label protein that engage the autophagy regulator MAP1LC3B (LC3/ATG8) in cells; the labeled proteins are then secreted, captured with neutravidin, tryptically digested, and identified by LC-MS/MS. Cells stably expressing BirA* alone serves as control for non-specific cytosolic labeling. SILAC is employed to quantify the degree of LC3B interaction over the background.
HostingRepository	PRIDE
AnnounceDate	2022-03-01
AnnouncementXML	Submission_2022-03-01_09:39:27.322.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hector Huang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	6x(13)C 4x(15)N labeled L-arginine; 6x(13)C 2x(15)N labeled L-lysine; phosphorylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2017-05-18 01:42:21	ID requested
⏵ 1	2022-03-01 09:39:28	announced

Leidal AM, Huang HH, Marsh T, Solvik T, Zhang D, Ye J, Kai F, Goldsmith J, Liu JY, Huang YH, Monkkonen T, Vlahakis A, Huang EJ, Goodarzi H, Yu L, Wiita AP, Debnath J, The LC3-conjugation machinery specifies the loading of RNA-binding proteins into extracellular vesicles. Nat Cell Biol, 22(2):187-199(2020) [pubmed]

curator keyword: Biological

submitter keyword: human, autophagy, MAP1LC3B, BioID, secretome, LC-MSMS, SILAC

Arun Paul Wiita
contact affiliation	Wiita Lab, Department of Laboratory Medicine, University of California San Francisco, USA
contact email	arun.wiita@ucsf.edu
lab head
Hector Huang
contact affiliation	UCSF
contact email	hector.huang@ucsf.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/03/PXD006541

PRIDE project URI

• PRIDE
  1. PXD006541
     1. Label: PRIDE project
     2. Name: LC-MSMS of cell secretome for MAP1LC3B interactors