PXD006534

PXD006534 is an original dataset announced via ProteomeXchange.

Title	HLA-DRB1*15:01 and HLA-DRB5*01:01 peptidomes
Description	HLA-DR15 is a haplotype associated with multiple sclerosis. It contains the two DRB* genes DRB1*1501 (DR2b) and DRB5*0101 (DR2a). The reported anchor motif of the corresponding HLA-DR molecules was determined years ago based on a small number of peptide ligands and binding assays. DR2a could display a set of peptides complementary to that presented by DR2b or, alternatively, a similar peptide repertoire but recognized in a different manner by T cells. It is known that DR2a and DR2b share some peptide ligands, although the degree of similarity of their associated peptidomes remains unclear. In addition, the contribution of each molecule to the global peptide repertoire presented by the HLA-DR15 haplotype has not been evaluated. We used mass spectrometry to analyze the peptide pools bound to DR2a and DR2b, identifying 169 and 555 unique peptide ligands of DR2a and DR2b, respectively. The analysis of these sets of peptides allowed the refinement of the corresponding binding motifs revealing novel anchor residues that had been overlooked in previous analyses. Moreover, the number of shared ligands between both molecules was low, indicating that DR2a and DR2b present complementary peptide repertoires to T cells. Finally, our analysis suggests that, quantitatively, both molecules contribute to the peptide repertoire presented by cells expressing the HLA-DR15 haplotype.
HostingRepository	PRIDE
AnnounceDate	2017-10-10
AnnouncementXML	Submission_2017-10-09_23:57:41.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Miguel Marcilla
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	2-pyrrolidone-5-carboxylic acid (Gln); monohydroxylated residue; acetylated residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2017-05-17 01:41:56	ID requested
⏵ 1	2017-10-09 23:57:42	announced

Scholz EM, Marcilla M, Daura X, Arribas-Layton D, James EA, Alvarez I, Human Leukocyte Antigen (HLA)-DRB1*15:01 and HLA-DRB5*01:01 Present Complementary Peptide Repertoires. Front Immunol, 8():984(2017) [pubmed]

curator keyword: Biomedical

submitter keyword: HLA-II, peptidome, HLA-DR15, DRB1*1501, DRB5*0101, Multiple Sclerosis

Iñaki Alvarez
contact affiliation	Institut de Biotecnologia i de Biomedicina, Universitat Autònoma de Barcelona, Spain
contact email	inaki.alvarez@uab.es
lab head
Miguel Marcilla
contact affiliation	Proteomics Unit - Dpt. of Macromolecular Structures - Spanish National Biotechnolgy Centre (CNB-CSIC)
contact email	mmarcilla@cnb.csic.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/10/PXD006534

PRIDE project URI

• PRIDE
  1. PXD006534
     1. Label: PRIDE project
     2. Name: HLA-DRB1*15:01 and HLA-DRB5*01:01 peptidomes