PXD006534 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | HLA-DRB1*15:01 and HLA-DRB5*01:01 peptidomes |
Description | HLA-DR15 is a haplotype associated with multiple sclerosis. It contains the two DRB* genes DRB1*1501 (DR2b) and DRB5*0101 (DR2a). The reported anchor motif of the corresponding HLA-DR molecules was determined years ago based on a small number of peptide ligands and binding assays. DR2a could display a set of peptides complementary to that presented by DR2b or, alternatively, a similar peptide repertoire but recognized in a different manner by T cells. It is known that DR2a and DR2b share some peptide ligands, although the degree of similarity of their associated peptidomes remains unclear. In addition, the contribution of each molecule to the global peptide repertoire presented by the HLA-DR15 haplotype has not been evaluated. We used mass spectrometry to analyze the peptide pools bound to DR2a and DR2b, identifying 169 and 555 unique peptide ligands of DR2a and DR2b, respectively. The analysis of these sets of peptides allowed the refinement of the corresponding binding motifs revealing novel anchor residues that had been overlooked in previous analyses. Moreover, the number of shared ligands between both molecules was low, indicating that DR2a and DR2b present complementary peptide repertoires to T cells. Finally, our analysis suggests that, quantitatively, both molecules contribute to the peptide repertoire presented by cells expressing the HLA-DR15 haplotype. |
HostingRepository | PRIDE |
AnnounceDate | 2017-10-10 |
AnnouncementXML | Submission_2017-10-09_23:57:41.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Miguel Marcilla |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | 2-pyrrolidone-5-carboxylic acid (Gln); monohydroxylated residue; acetylated residue |
Instrument | TripleTOF 5600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2017-05-17 01:41:56 | ID requested | |
⏵ 1 | 2017-10-09 23:57:42 | announced | |
Publication List
Keyword List
curator keyword: Biomedical |
submitter keyword: HLA-II, peptidome, HLA-DR15, DRB1*1501, DRB5*0101, Multiple Sclerosis |
Contact List
Iñaki Alvarez |
contact affiliation | Institut de Biotecnologia i de Biomedicina, Universitat Autònoma de Barcelona, Spain |
contact email | inaki.alvarez@uab.es |
lab head | |
Miguel Marcilla |
contact affiliation | Proteomics Unit - Dpt. of Macromolecular Structures - Spanish National Biotechnolgy Centre (CNB-CSIC) |
contact email | mmarcilla@cnb.csic.es |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD006534
- Label: PRIDE project
- Name: HLA-DRB1*15:01 and HLA-DRB5*01:01 peptidomes