PXD006531

PXD006531 is an original dataset announced via ProteomeXchange.

Title	Proteome of an early evolutionary stage photosynthetic organelle in the amoeba Paulinella chromatophora
Description	The endosymbiotic acquisition of mitochondria and plastids >1 Ga ago profoundly impacted eukaryote evolution. Early stages of organelle integration however remain poorly understood. The amoeba Paulinella chromatophora contains more recently established cyanobacterium-derived photosynthetic organelles, termed “chromatophores”. To explore the re-arrangement of an organellar proteome during its integration into a eukaryotic host cell, we characterized the chromatophore proteome by mass spectrometry. Apparently, genetic control over the chromatophore has shifted substantially to the nucleus. Two classes of nuclear-encoded proteins are imported into the chromatophore, likely through independent pathways. Most imported proteins seem to derive from ancestral host genes rather than nuclear genes transferred from the endosymbiont. Intriguingly, the putative targeting signal found in one class of imported proteins confers chloroplast localization upon heterologous expression in a plant cell suggesting common features in chromatophore and plastid protein import pathways. Finally, combining experimental data with in silico predictions we provide a comprehensive catalogue of chromatophore-localized proteins.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:38:05.997.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gereon Poschmann
SpeciesList	scientific name: Paulinella chromatophora; NCBI TaxID: 39717;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-05-16 06:44:38	ID requested
1	2017-07-13 04:03:31	announced
2	2017-09-25 02:19:35	announced	Updated publication reference for PubMed record(s): 28889978.
⏵ 3	2024-10-22 04:38:14	announced	2024-10-22: Updated project metadata.

Singer A, Poschmann G, M, ü, hlich C, Valadez-Cano C, H, ä, nsch S, H, ü, ren V, Rensing SA, St, ü, hler K, Nowack ECM, Massive Protein Import into the Early-Evolutionary-Stage Photosynthetic Organelle of the Amoeba Paulinella chromatophora. Curr Biol, 27(18):2763-2773.e5(2017) [pubmed]

10.1016/j.cub.2017.08.010;

curator keyword: Biological

submitter keyword: organell evolution, evolution,Model organism, endosymbiosis

Gereon Poschmann
contact affiliation	Molecular Proteomics Laboratory (MPL) Biologisch-Medizinisches Forschungszentrum (BMFZ) Heinrich-Heine-Universität Düsseldorf 40225 Düsseldorf Germany
contact email	gereon.poschmann@hhu.de
lab head
Gereon Poschmann
contact affiliation	Molecular Proteomics Laboratory
contact email	gereon.poschmann@hhu.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD006531
     1. Label: PRIDE project
     2. Name: Proteome of an early evolutionary stage photosynthetic organelle in the amoeba Paulinella chromatophora