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PXD006531

PXD006531 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteome of an early evolutionary stage photosynthetic organelle in the amoeba Paulinella chromatophora
DescriptionThe endosymbiotic acquisition of mitochondria and plastids >1 Ga ago profoundly impacted eukaryote evolution. Early stages of organelle integration however remain poorly understood. The amoeba Paulinella chromatophora contains more recently established cyanobacterium-derived photosynthetic organelles, termed “chromatophores”. To explore the re-arrangement of an organellar proteome during its integration into a eukaryotic host cell, we characterized the chromatophore proteome by mass spectrometry. Apparently, genetic control over the chromatophore has shifted substantially to the nucleus. Two classes of nuclear-encoded proteins are imported into the chromatophore, likely through independent pathways. Most imported proteins seem to derive from ancestral host genes rather than nuclear genes transferred from the endosymbiont. Intriguingly, the putative targeting signal found in one class of imported proteins confers chloroplast localization upon heterologous expression in a plant cell suggesting common features in chromatophore and plastid protein import pathways. Finally, combining experimental data with in silico predictions we provide a comprehensive catalogue of chromatophore-localized proteins.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:38:05.997.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterGereon Poschmann
SpeciesList scientific name: Paulinella chromatophora; NCBI TaxID: 39717;
ModificationListmonohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02017-05-16 06:44:38ID requested
12017-07-13 04:03:31announced
22017-09-25 02:19:35announcedUpdated publication reference for PubMed record(s): 28889978.
32024-10-22 04:38:14announced2024-10-22: Updated project metadata.
Publication List
Singer A, Poschmann G, M, ü, hlich C, Valadez-Cano C, H, ä, nsch S, H, ü, ren V, Rensing SA, St, ü, hler K, Nowack ECM, Massive Protein Import into the Early-Evolutionary-Stage Photosynthetic Organelle of the Amoeba Paulinella chromatophora. Curr Biol, 27(18):2763-2773.e5(2017) [pubmed]
10.1016/j.cub.2017.08.010;
Keyword List
curator keyword: Biological
submitter keyword: organell evolution, evolution,Model organism, endosymbiosis
Contact List
Gereon Poschmann
contact affiliationMolecular Proteomics Laboratory (MPL) Biologisch-Medizinisches Forschungszentrum (BMFZ) Heinrich-Heine-Universität Düsseldorf 40225 Düsseldorf Germany
contact emailgereon.poschmann@hhu.de
lab head
Gereon Poschmann
contact affiliationMolecular Proteomics Laboratory
contact emailgereon.poschmann@hhu.de
dataset submitter
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