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PXD006528

PXD006528 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomic characterization of Withaferin A-targeted protein networks for the treatment of monoclonal myeloma gammopathies
DescriptionWithaferin A (WA), a natural steroid lactone from the plant Withania Somnifera, has been used for many years in traditional Ayurveda medicine to treat inflammation-related conditions, such as ulcers and rheumatism. Today, WA is most often studied because of its antitumor properties. Although many in vitro and in vivo studies have been performed, the identification of Withaferin A protein targets and of its mechanism of antitumor action remains incomplete. Here, we used quantitative chemoproteomics and differential protein expression analysis to characterize the WA antitumor effects on a multiple myeloma cell model. Identified relevant targets were further validated by Ingenuity Pathway Analysis (IPA) and Western Blot. Canonical pathway analysis revealed targeting of the ubiquitination pathway as a main strategy for WA-induced cell death. Further data suggested that WA targets protein networks that are specific for monoclonal gammopathy of undetermined significance (MGUS) and other closely related disorders, such as multiple myeloma (MM) and Waldenström macroglobulinemia (WM). Moreover, 16 of 20 proteins influenced by WA potentially counteract disease progression, suggesting WA can be used as an effective treatment for MGUS and other closely related disorders.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:37:53.690.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD006528
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmittermartin dom
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListCation:Li; Amidated; Oxidation; Label:13C(6); Carbamidomethyl
InstrumentLTQ Orbitrap Velos; 4800 Proteomics Analyzer
Dataset History
RevisionDatetimeStatusChangeLog Entry
02017-05-16 05:48:46ID requested
12018-02-22 19:33:15announced
22024-10-22 04:37:57announced2024-10-22: Updated project metadata.
Publication List
10.6019/PXD006528;
10.1016/j.jprot.2018.02.013;
Dom M, Offner F, Vanden Berghe W, Van Ostade X, Proteomic characterization of Withaferin A-targeted protein networks for the treatment of monoclonal myeloma gammopathies. J Proteomics, 179():17-29(2018) [pubmed]
Keyword List
curator keyword: Biomedical
submitter keyword: Withaferin A, quantitative chemoproteomics, Quantitative differential expression analysis, multiple myeloma
Contact List
Xaveer Van Ostade
contact affiliationLaboratory of Protein Chemistry, Proteomics and Epigenetic Signalling (PPES), Department of Biomedical Sciences, University of Antwerp (UA), Belgium
contact emailxaveer.vanostade@uantwerpen.be
lab head
martin dom
contact affiliationUniversity of Antwerp
contact emailmartin.dom@uantwerpen.be
dataset submitter
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