PXD006528

PXD006528 is an original dataset announced via ProteomeXchange.

Title	Proteomic characterization of Withaferin A-targeted protein networks for the treatment of monoclonal myeloma gammopathies
Description	Withaferin A (WA), a natural steroid lactone from the plant Withania Somnifera, has been used for many years in traditional Ayurveda medicine to treat inflammation-related conditions, such as ulcers and rheumatism. Today, WA is most often studied because of its antitumor properties. Although many in vitro and in vivo studies have been performed, the identification of Withaferin A protein targets and of its mechanism of antitumor action remains incomplete. Here, we used quantitative chemoproteomics and differential protein expression analysis to characterize the WA antitumor effects on a multiple myeloma cell model. Identified relevant targets were further validated by Ingenuity Pathway Analysis (IPA) and Western Blot. Canonical pathway analysis revealed targeting of the ubiquitination pathway as a main strategy for WA-induced cell death. Further data suggested that WA targets protein networks that are specific for monoclonal gammopathy of undetermined significance (MGUS) and other closely related disorders, such as multiple myeloma (MM) and Waldenström macroglobulinemia (WM). Moreover, 16 of 20 proteins influenced by WA potentially counteract disease progression, suggesting WA can be used as an effective treatment for MGUS and other closely related disorders.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:37:53.690.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD006528
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	martin dom
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Cation:Li; Amidated; Oxidation; Label:13C(6); Carbamidomethyl
Instrument	LTQ Orbitrap Velos; 4800 Proteomics Analyzer

Revision	Datetime	Status	ChangeLog Entry
0	2017-05-16 05:48:46	ID requested
1	2018-02-22 19:33:15	announced
⏵ 2	2024-10-22 04:37:57	announced	2024-10-22: Updated project metadata.

10.6019/PXD006528;

10.1016/j.jprot.2018.02.013;

Dom M, Offner F, Vanden Berghe W, Van Ostade X, Proteomic characterization of Withaferin A-targeted protein networks for the treatment of monoclonal myeloma gammopathies. J Proteomics, 179():17-29(2018) [pubmed]

curator keyword: Biomedical

submitter keyword: Withaferin A, quantitative chemoproteomics, Quantitative differential expression analysis, multiple myeloma

Xaveer Van Ostade
contact affiliation	Laboratory of Protein Chemistry, Proteomics and Epigenetic Signalling (PPES), Department of Biomedical Sciences, University of Antwerp (UA), Belgium
contact email	xaveer.vanostade@uantwerpen.be
lab head
martin dom
contact affiliation	University of Antwerp
contact email	martin.dom@uantwerpen.be
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD006528
     1. Label: PRIDE project
     2. Name: Proteomic characterization of Withaferin A-targeted protein networks for the treatment of monoclonal myeloma gammopathies