PXD006527

PXD006527 is an original dataset announced via ProteomeXchange.

Title	A thiol probe for measuring unfolded protein load and proteostasis in cells
Description	When proteostasis becomes unbalanced, unfolded proteins accumulate and can aggregate. However, probes quantifying proteostasis imbalance are lacking. We report a dye, tetraphenylethene maleimide (TPE-MI) that can measure unfolded protein load.TPE-MI fluorescence is enhanced upon reaction with cellular proteomes under conditions promoting accumulation of unfolded proteins and, via proteomic analysis, can track which proteins expose more cysteine residues under stress.
HostingRepository	PRIDE
AnnounceDate	2018-10-24
AnnouncementXML	Submission_2018-10-24_12:52:17.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD006527
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Nagaraj Moily
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	Oxidation; Acetyl; Carbamidomethyl; Oxidation
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2017-05-16 04:14:21	ID requested
⏵ 1	2018-10-24 12:52:18	announced

Chen MZ, Moily NS, Bridgford JL, Wood RJ, Radwan M, Smith TA, Song Z, Tang BZ, Tilley L, Xu X, Reid GE, Pouladi MA, Hong Y, Hatters DM, A thiol probe for measuring unfolded protein load and proteostasis in cells. Nat Commun, 8(1):474(2017) [pubmed]

curator keyword: Biological

submitter keyword: Mouse, Neuro2a, TPE-MI, SILAC, LC/MSMS, Orbitrap Fusion Lumos

Danny Hatters
contact affiliation	Department of Biochemistry and Molecular Biology and Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Parkville, VIC 3010, Australia
contact email	dhatters@unimelb.edu.au
lab head
Nagaraj Moily
contact affiliation	The University of Melbourne
contact email	nagaraj.s.moily@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD006527
     1. Label: PRIDE project
     2. Name: A thiol probe for measuring unfolded protein load and proteostasis in cells