PXD006525

PXD006525 is an original dataset announced via ProteomeXchange.

Title	Quantitative Proteomic Atlas Uncovering the widespread Ubiquitylation Regulation in the Embryo of Germinating Rice Seed
Description	Using high affinity K-Ɛ-GG antibody integrating highly sensitive MS, we investigated the quantitative atlas of ubiquitylome as well as the proteome in the embryos of germinated rice seed. A list of 2576 lysine ubiquitinated sites in 1171 proteins,mainly involved in protein process, nucleic acid stability, hormonal regulation, and metabolism,were identified in the first 0, 12 and 24 hours after seed imbibition (HAI). The additional ubiquitin were more likely to attacked the lysine sites that located in the polar acidic other than basophilic regions. Of these modified proteins,1435 sites in 781 proteins were significantly changed in abundance (folds change> 2, P<0.05), most ubiquitinated sites were increased rapidly in the first 12 HAI.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:33:31.475.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dongli He
SpeciesList	scientific name: Oryza sativa (Rice); NCBI TaxID: 4530;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-05-16 03:19:29	ID requested
1	2022-02-23 08:49:26	announced
⏵ 2	2024-10-22 05:33:31	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

curator keyword: Biological

submitter keyword: Rice, Seed Germination,lysine ubiquitylation, Quantitative proteome, Embryo

Pingfang Yang
contact affiliation	Aquatic Plant Genomics and Genetic Breeding, Wuhan Botanical Garden
contact email	yangpf@wbgcas.cn
lab head
Dongli He
contact affiliation	Wuhan Botanic Garden
contact email	hedongli@wbgcas.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD006525

PRIDE project URI

• PRIDE
  1. PXD006525
     1. Label: PRIDE project
     2. Name: Quantitative Proteomic Atlas Uncovering the widespread Ubiquitylation Regulation in the Embryo of Germinating Rice Seed