PXD006510

PXD006510 is an original dataset announced via ProteomeXchange.

Title	Crosslinking mass spectrometry of yeast Pol I pre-initiation complex
Description	In eukaryotic cells, ribosomal RNA is transcribed by RNA polymerase I (Pol I). To initiate transcription, Pol I requires the assembly of a multi-subunit pre-initiation complex (PIC). In yeast, the minimal PIC includes Pol I, the transcription factor Rrn3 and Core Factor (CF) composed of subunits Rrn6, Rrn7 and Rrn11. We have determined the cryo-EM structure of a 18-subunit yeast Pol I complex together with CF, Rrn3 and transcription scaffold. To aid in modeling the structure based on the cryo-EM map, we performed crosslinking mass spectrometry.
HostingRepository	PRIDE
AnnounceDate	2017-07-31
AnnouncementXML	Submission_2017-07-31_02:51:42.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Katarzyna Buczak
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2017-05-12 08:03:41	ID requested
⏵ 1	2017-07-31 02:51:43	announced

Sadian Y, Tafur L, Kosinski J, Jakobi AJ, Wetzel R, Buczak K, Hagen WJ, Beck M, Sachse C, M, ü, ller CW, Structural insights into transcription initiation by yeast RNA polymerase I. EMBO J, 36(18):2698-2709(2017) [pubmed]

curator keyword: Biological

submitter keyword: Pol I pre-initiation complex, Pol I, Core factor, ribosomal RNA, transcription, crosslinking

Christoph Müller
contact affiliation	European Molecular Biology Laboratory (EMBL), Structural and Computational Biology Unit, Meyerhofstrasse 1, 69117 Heidelberg, Germany
contact email	christoph.mueller@embl.de
lab head
Katarzyna Buczak
contact affiliation	European Molecular Biology Laboratory
contact email	buczak@embl.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD006510
     1. Label: PRIDE project
     2. Name: Crosslinking mass spectrometry of yeast Pol I pre-initiation complex