PXD006501 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Quantitative proteomic analysis of the human extracellular vesicle proteome reveals a novel mechanism for tissue cross-talk during exercise |
Description | Exercise stimulates the release of a plethora of molecules into the circulation, supporting the concept that inter-tissue signaling proteins are important mediators of adaptations to exercise. Recognising that many circulating proteins might be packaged in extracellular vesicles (EV), we employed quantitative proteomic techniques to characterise the exercise-induced secretion of EV contained proteins. We observed an increase in circulation of over 300 proteins, with a notable enrichment of several classes of proteins that compose exosomes and small vesicles, in human arterial blood samples. Pathway analyses revealed significant enrichments in a multitude of biological processes and signalling pathways. Pulse chase and intravital imaging experiments suggest EV-mediated muscle-liver cross talk during exercise. Moreover, by employing arterio-venous balance studies across the contracting human limb, we identified several novel candidate myokines. These data offer a new paradigm by which tissue cross-talk during exercise can exert systemic biological effects |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:37:31.051.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Martin Whitham |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2017-05-12 00:29:22 | ID requested | |
1 | 2018-01-10 00:45:15 | announced | |
2 | 2018-01-19 06:45:24 | announced | Updated publication reference for PubMed record(s): 29320704. |
⏵ 3 | 2024-10-22 04:37:33 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1016/j.cmet.2017.12.001; |
Whitham M, Parker BL, Friedrichsen M, Hingst JR, Hjorth M, Hughes WE, Egan CL, Cron L, Watt KI, Kuchel RP, Jayasooriah N, Estevez E, Petzold T, Suter CM, Gregorevic P, Kiens B, Richter EA, James DE, Wojtaszewski JFP, Febbraio MA, Extracellular Vesicles Provide a Means for Tissue Crosstalk during Exercise. Cell Metab, 27(1):237-251.e4(2018) [pubmed] |
Keyword List
curator keyword: Biological, Biomedical |
submitter keyword: Muscle,Exercise, Integrin, Myokine, Arterio-venous, Exosome, Extracellular Vesicle, Proteomics |
Contact List
Martin Whitham |
contact affiliation | Diabetes & Metabolism, Garvan Institute of Medical Research |
contact email | martwhitham@gmail.com |
lab head | |
Martin Whitham |
contact affiliation | Garvan Institute of Medical Research |
contact email | martwhitham@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/01/PXD006501 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD006501
- Label: PRIDE project
- Name: Quantitative proteomic analysis of the human extracellular vesicle proteome reveals a novel mechanism for tissue cross-talk during exercise