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PXD006501

PXD006501 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleQuantitative proteomic analysis of the human extracellular vesicle proteome reveals a novel mechanism for tissue cross-talk during exercise
DescriptionExercise stimulates the release of a plethora of molecules into the circulation, supporting the concept that inter-tissue signaling proteins are important mediators of adaptations to exercise. Recognising that many circulating proteins might be packaged in extracellular vesicles (EV), we employed quantitative proteomic techniques to characterise the exercise-induced secretion of EV contained proteins. We observed an increase in circulation of over 300 proteins, with a notable enrichment of several classes of proteins that compose exosomes and small vesicles, in human arterial blood samples. Pathway analyses revealed significant enrichments in a multitude of biological processes and signalling pathways. Pulse chase and intravital imaging experiments suggest EV-mediated muscle-liver cross talk during exercise. Moreover, by employing arterio-venous balance studies across the contracting human limb, we identified several novel candidate myokines. These data offer a new paradigm by which tissue cross-talk during exercise can exert systemic biological effects
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:37:31.051.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMartin Whitham
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02017-05-12 00:29:22ID requested
12018-01-10 00:45:15announced
22018-01-19 06:45:24announcedUpdated publication reference for PubMed record(s): 29320704.
32024-10-22 04:37:33announced2024-10-22: Updated project metadata.
Publication List
10.1016/j.cmet.2017.12.001;
Whitham M, Parker BL, Friedrichsen M, Hingst JR, Hjorth M, Hughes WE, Egan CL, Cron L, Watt KI, Kuchel RP, Jayasooriah N, Estevez E, Petzold T, Suter CM, Gregorevic P, Kiens B, Richter EA, James DE, Wojtaszewski JFP, Febbraio MA, Extracellular Vesicles Provide a Means for Tissue Crosstalk during Exercise. Cell Metab, 27(1):237-251.e4(2018) [pubmed]
Keyword List
curator keyword: Biological, Biomedical
submitter keyword: Muscle,Exercise, Integrin, Myokine, Arterio-venous, Exosome, Extracellular Vesicle, Proteomics
Contact List
Martin Whitham
contact affiliationDiabetes & Metabolism, Garvan Institute of Medical Research
contact emailmartwhitham@gmail.com
lab head
Martin Whitham
contact affiliationGarvan Institute of Medical Research
contact emailmartwhitham@gmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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