PXD006501

PXD006501 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteomic analysis of the human extracellular vesicle proteome reveals a novel mechanism for tissue cross-talk during exercise
Description	Exercise stimulates the release of a plethora of molecules into the circulation, supporting the concept that inter-tissue signaling proteins are important mediators of adaptations to exercise. Recognising that many circulating proteins might be packaged in extracellular vesicles (EV), we employed quantitative proteomic techniques to characterise the exercise-induced secretion of EV contained proteins. We observed an increase in circulation of over 300 proteins, with a notable enrichment of several classes of proteins that compose exosomes and small vesicles, in human arterial blood samples. Pathway analyses revealed significant enrichments in a multitude of biological processes and signalling pathways. Pulse chase and intravital imaging experiments suggest EV-mediated muscle-liver cross talk during exercise. Moreover, by employing arterio-venous balance studies across the contracting human limb, we identified several novel candidate myokines. These data offer a new paradigm by which tissue cross-talk during exercise can exert systemic biological effects
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:37:31.051.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Martin Whitham
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-05-12 00:29:22	ID requested
1	2018-01-10 00:45:15	announced
2	2018-01-19 06:45:24	announced	Updated publication reference for PubMed record(s): 29320704.
⏵ 3	2024-10-22 04:37:33	announced	2024-10-22: Updated project metadata.

10.1016/j.cmet.2017.12.001;

Whitham M, Parker BL, Friedrichsen M, Hingst JR, Hjorth M, Hughes WE, Egan CL, Cron L, Watt KI, Kuchel RP, Jayasooriah N, Estevez E, Petzold T, Suter CM, Gregorevic P, Kiens B, Richter EA, James DE, Wojtaszewski JFP, Febbraio MA, Extracellular Vesicles Provide a Means for Tissue Crosstalk during Exercise. Cell Metab, 27(1):237-251.e4(2018) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: Muscle,Exercise, Integrin, Myokine, Arterio-venous, Exosome, Extracellular Vesicle, Proteomics

Martin Whitham
contact affiliation	Diabetes & Metabolism, Garvan Institute of Medical Research
contact email	martwhitham@gmail.com
lab head
Martin Whitham
contact affiliation	Garvan Institute of Medical Research
contact email	martwhitham@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/01/PXD006501

PRIDE project URI

• PRIDE
  1. PXD006501
     1. Label: PRIDE project
     2. Name: Quantitative proteomic analysis of the human extracellular vesicle proteome reveals a novel mechanism for tissue cross-talk during exercise