PXD006498

PXD006498 is an original dataset announced via ProteomeXchange.

Title	N6-methyl adenosine (m6A) recruits and repels proteins to regulate mRNA homeostasis
Description	RNA modifications are integral to regulation of RNA metabolism. One such abundant mRNA modification is m6A, which impacts various aspects of RNA metabolism including splicing, transport and degradation. Current knowledge about proteins recruited to m6A to carry out these molecular processes is still limited. Here we describe a comprehensive and systematic mass spectrometry-based screening of m6A interactors in various cell types and species. Amongst the main findings, we identified G3BP1 as a protein, which is repelled by m6A and which positively regulates mRNA stability in an m6A regulated manner. Furthermore, we identified FMR1 as a novel, RNA sequence context dependent m6A reader, thus revealing a connection between an mRNA modification and an autism spectrum disorder. Collectively, our data represents a rich resource for the community and sheds further light on the complex interplay between m6A, m6A interactors and mRNA homeostasis.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:38:12.313.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rik Lindeboom
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-05-11 08:30:13	ID requested
1	2017-08-22 07:02:24	announced
2	2017-09-06 23:51:20	announced	Updated publication reference for PubMed record(s): 28869609.
⏵ 3	2024-10-22 04:38:15	announced	2024-10-22: Updated project metadata.

Edupuganti RR, Geiger S, Lindeboom RGH, Shi H, Hsu PJ, Lu Z, Wang SY, Baltissen MPA, Jansen PWTC, Rossa M, M, ü, ller M, Stunnenberg HG, He C, Carell T, Vermeulen M, A) recruits and repels proteins to regulate mRNA homeostasis. Nat Struct Mol Biol, 24(10):870-878(2017) [pubmed]

10.1038/nsmb.3462;

curator keyword: Biological

submitter keyword: interaction proteomics, FMR1,m6A, G3BP1, mRNA homeostasis

Michiel Vermeulen
contact affiliation	Department of Molecular Biology, Faculty of Science, Radboud Institute for Molecular Life Sciences, Radboud University Nijmegen, Nijmegen 6525, the Netherlands.
contact email	michiel.vermeulen@science.ru.nl
lab head
Rik Lindeboom
contact affiliation	Radboud Institute for Molecular Life Sciences
contact email	r.lindeboom@science.ru.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/08/PXD006498

PRIDE project URI

• PRIDE
  1. PXD006498
     1. Label: PRIDE project
     2. Name: N6-methyl adenosine (m6A) recruits and repels proteins to regulate mRNA homeostasis