PXD006484

PXD006484 is an original dataset announced via ProteomeXchange.

Title	Immune repertoire after immunization as seen by next generation sequencing and proteomics
Description	We study the repertoire of immunoglobulin sequences after immunization in a cohort of rats. Animals were immunized with dinitrophenol modified KLH or with recombinant human HuD. In the immune repertoire, and also in the matching proteomics data, we observe that the animals produce a repertoire that has many shared motifs for those immunized with the same antigen. Cluster analysis allows the samples to be segregated according to the immunogen used.
HostingRepository	PRIDE
AnnounceDate	2017-10-04
AnnouncementXML	Submission_2017-11-06_00:49:56.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD006484
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Martijn van Duijn
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	Oxidation; Carbamidomethyl
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2017-05-10 06:29:36	ID requested
1	2017-10-04 06:05:38	announced
⏵ 2	2017-11-06 00:49:57	announced	Updated publication reference for PubMed record(s): 29085363, 20615873.

VanDuijn MM, Dekker LJ, Zeneyedpour L, Smitt PA, Luider TM, Immune responses are characterized by specific shared immunoglobulin peptides that can be detected by proteomic techniques. J Biol Chem, 285(38):29247-53(2010) [pubmed]

VanDuijn MM, Dekker LJ, van IJcken WFJ, Sillevis Smitt PAE, Luider TM, Immune Repertoire after Immunization As Seen by Next-Generation Sequencing and Proteomics. Front Immunol, 8():1286(2017) [pubmed]

curator keyword: Biological

submitter keyword: Immune repertoire, immunization, NGS, mass spectrometry, immunoglobulins, proteogenomics

Theo Luider
contact affiliation	Erasmus MC Department of Neurology Clinical and Cancer Proteomics
contact email	t.luider@erasmusmc.nl
lab head
Martijn van Duijn
contact affiliation	Erasmus MC
contact email	m.m.vanduijn@erasmusmc.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/10/PXD006484

PRIDE project URI

• PRIDE
  1. PXD006484
     1. Label: PRIDE project
     2. Name: Immune repertoire after immunization as seen by next generation sequencing and proteomics