PXD006468

PXD006468 is an original dataset announced via ProteomeXchange.

Title	Multi-stress experiment on Pseudonitzschia multiseries
Description	A changing climate is altering many ocean properties that consequently will modify marine productivity. Previous phytoplankton manipulation studies have focused on individual or subsets of these properties. Here, we investigate the cumulative effects of multi-faceted change on a subantarctic diatom Pseudonitzschia multiseries by concurrently manipulating five stressors (light/nutrients/CO2/temperature/iron) that primarily control its physiology, and explore underlying reasons for altered physiological performance. Climate change enhances diatom growth mainly owing to warming and iron enrichment, and both properties decrease cellular nutrient quotas, partially offsetting any effects of decreased nutrient supply by 2100. Physiological diagnostics and comparative proteomics demonstrate the joint importance of individual and interactive effects of temperature and iron, and reveal biased future predictions from experimental outcomes when only a subset of multi-stressors is considered. Our findings for subantarctic waters illustrate how composite regional studies are needed to provide accurate global projections of future shifts in productivity and distinguish underlying species-specific physiological mechanisms.
HostingRepository	PRIDE
AnnounceDate	2017-05-19
AnnouncementXML	Submission_2018-05-23_05:45:23.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Brook Nunn
SpeciesList	scientific name: Pseudo-nitzschia multiseries; NCBI TaxID: 37319;
ModificationList	monohydroxylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-05-09 23:28:57	ID requested
1	2017-05-19 02:31:35	announced
⏵ 2	2018-05-23 05:45:24	announced	Updated publication reference for DOI(s): 10.1038/nclimate2811.

10.1038/nclimate2811;

curator keyword: Biological

submitter keyword: incubation, proteomics, climate change, iron, light, salinity, multi-stress incubation, Pseudonitzschia multiseries

Brook L. Nuun
contact affiliation	University of Washington, Department of Genome Sciences
contact email	brookh@uw.edu
lab head
Brook Nunn
contact affiliation	University of Washington
contact email	brookh@uw.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/05/PXD006468

PRIDE project URI

• PRIDE
  1. PXD006468
     1. Label: PRIDE project
     2. Name: Multi-stress experiment on Pseudonitzschia multiseries