PXD006467

PXD006467 is an original dataset announced via ProteomeXchange.

Title	Oxygen-dependent changes in protein expression in Campylobacter jejuni NCTC11168
Description	Using strain NCTC 11168 in carbon (serine)-limited continuous cultures we report the first detailed examination of oxygen-dependent changes in gene and protein expression in Campylobacter jejuni under conditions where the growth rate is fixed. We show that in steady-states established at μ = 0.2 h-1 over a wide-range of oxygen inputs, a perceived aerobiosis scale can be calibrated by the acetate excretion flux, which becomes zero when metabolism is fully aerobic (100% aerobiosis = 5% v/v oxygen in the gas inflow). A label-free proteomic analysis compared protein abundance (reported as the emPAI ratio) at 40% aerobiosis (= 1.88% v/v oxygen in the gas inflow) and 150% aerobiosis (= 7.5% v/v oxygen in the gas inflow) aerobiosis. This identified 857 proteins. Of 223 proteins more abundant at 40% aerobiosis, those involved in host colonisation and alternative pathways of electron transport were prominent. At 150% aerobiosis, 129 proteins were more abundant, including those involved in oxidative stress protection, citric-acid cycle, lactate and proline oxidation.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:37:04.028.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD006467
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	David Kelly
SpeciesList	scientific name: Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819; NCBI TaxID: 192222;
ModificationList	Oxidation; Carbamidomethyl
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2017-05-09 23:27:32	ID requested
1	2017-10-04 02:17:19	announced
⏵ 2	2024-10-22 04:37:04	announced	2024-10-22: Updated project metadata.

10.1111/1462-2920.13930;

10.6019/PXD006467;

Guccione EJ, Kendall JJ, Hitchcock A, Garg N, White MA, Mulholland F, Poole RK, Kelly DJ, Transcriptome and proteome dynamics in chemostat culture reveal how Campylobacter jejuni modulates metabolism, stress responses and virulence factors upon changes in oxygen availability. Environ Microbiol, 19(10):4326-4348(2017) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: oxygen, electron transport, mass spectrometry, oxidative stress, label-free,Campylobacter

Professor David Jacob Kelly
contact affiliation	Department of Molecular Biology and Biotechnology The University of Sheffield Sheffield S10 2TN, UK
contact email	d.kelly@sheffield.ac.uk
lab head
David Kelly
contact affiliation	University of Sheffield
contact email	d.kelly@sheffield.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD006467
     1. Label: PRIDE project
     2. Name: Oxygen-dependent changes in protein expression in Campylobacter jejuni NCTC11168