⮝ Full datasets listing

PXD006467

PXD006467 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleOxygen-dependent changes in protein expression in Campylobacter jejuni NCTC11168
DescriptionUsing strain NCTC 11168 in carbon (serine)-limited continuous cultures we report the first detailed examination of oxygen-dependent changes in gene and protein expression in Campylobacter jejuni under conditions where the growth rate is fixed. We show that in steady-states established at μ = 0.2 h-1 over a wide-range of oxygen inputs, a perceived aerobiosis scale can be calibrated by the acetate excretion flux, which becomes zero when metabolism is fully aerobic (100% aerobiosis = 5% v/v oxygen in the gas inflow). A label-free proteomic analysis compared protein abundance (reported as the emPAI ratio) at 40% aerobiosis (= 1.88% v/v oxygen in the gas inflow) and 150% aerobiosis (= 7.5% v/v oxygen in the gas inflow) aerobiosis. This identified 857 proteins. Of 223 proteins more abundant at 40% aerobiosis, those involved in host colonisation and alternative pathways of electron transport were prominent. At 150% aerobiosis, 129 proteins were more abundant, including those involved in oxidative stress protection, citric-acid cycle, lactate and proline oxidation.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:37:04.028.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD006467
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterDavid Kelly
SpeciesList scientific name: Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819; NCBI TaxID: 192222;
ModificationListOxidation; Carbamidomethyl
InstrumentLTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02017-05-09 23:27:32ID requested
12017-10-04 02:17:19announced
22024-10-22 04:37:04announced2024-10-22: Updated project metadata.
Publication List
10.1111/1462-2920.13930;
10.6019/PXD006467;
Guccione EJ, Kendall JJ, Hitchcock A, Garg N, White MA, Mulholland F, Poole RK, Kelly DJ, Transcriptome and proteome dynamics in chemostat culture reveal how Campylobacter jejuni modulates metabolism, stress responses and virulence factors upon changes in oxygen availability. Environ Microbiol, 19(10):4326-4348(2017) [pubmed]
Keyword List
curator keyword: Biological, Biomedical
submitter keyword: oxygen, electron transport, mass spectrometry, oxidative stress, label-free,Campylobacter
Contact List
Professor David Jacob Kelly
contact affiliationDepartment of Molecular Biology and Biotechnology The University of Sheffield Sheffield S10 2TN, UK
contact emaild.kelly@sheffield.ac.uk
lab head
David Kelly
contact affiliationUniversity of Sheffield
contact emaild.kelly@sheffield.ac.uk
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/10/PXD006467
PRIDE project URI
Repository Record List
[ + ]