PXD006465

PXD006465 is an original dataset announced via ProteomeXchange.

Title	Human Testis LC-MS/MS
Description	Missing proteins(MPs) investigation is one of the critical missions of Chromosome-based Human Proteome Project(C-HPP). Through analyzing sequence of MPs from neXtProt database, we found that there are no detectable tryptic peptides for some MPs. Here, we attmept a multi-enzymes strategy to cover this problem. Lys-C, Glu-C and LysargiNase are used to produce more unique and detectable peptides of MPs.
HostingRepository	PRIDE
AnnounceDate	2017-10-09
AnnouncementXML	Submission_2017-10-09_02:13:52.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD006465
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Yihao Wang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2017-05-08 02:34:34	ID requested
⏵ 1	2017-10-09 02:13:54	announced

Wang Y, Chen Y, Zhang Y, Wei W, Li Y, Zhang T, He F, Gao Y, Xu P, Multi-Protease Strategy Identifies Three PE2 Missing Proteins in Human Testis Tissue. J Proteome Res, 16(12):4352-4363(2017) [pubmed]

ProteomeXchange project tag: Chromosome-centric Human Proteome Project (C-HPP), Human Proteome Project

curator keyword: Biological

submitter keyword: Testis, LC-MS/MS, Multi-enzymes

Ping Xu
contact affiliation	Secretary General of CNHUPO Professor, Director Department of Genomics and Proteomics, State Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences Beijing Beijing Institute of Radiation Medicine， 38 Life Science Park Road, Beijing 102206, P.R. China.
contact email	xupingghy@gmail.com
lab head
Yihao Wang
contact affiliation	NCPSB
contact email	hao_god@126.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/10/PXD006465

PRIDE project URI

• PRIDE
  1. PXD006465
     1. Label: PRIDE project
     2. Name: Human Testis LC-MS/MS