PXD006462

PXD006462 is an original dataset announced via ProteomeXchange.

Title	Identification of molecular response to blood meal in the fatbody of Anopheles stephensi using quantitative proteomics
Description	Fat body is an important tissue in the context of vitellogenesis, vector immunity, vector physiology and vector-parasite interaction. However, the proteome of fatbody and impact of blood meal on the gene expression of this vital organ has not been investigated so far. Therefore, in this study, we made an attempt to identify proteins expressed in fatbody of An. stephensi and their altered expression in response to blood meal. In all, we identified 4,504 proteins in the fatbody using multiple fractionation strategies, which is by far the largest resource of fatbody proteome in any mosquito species. Further, comparative proteomic analysis of fatbody 24 and 48 hours post blood meal led to identification of over 300 differentially expressed proteins. Bioinformatics analysis of these proteins suggested their role in vitellogenesis, lipid transport, mosquito immunity and oxidation-reduction processes. Interestingly, we identified four novel genes,which were found to be differentially expressed upon blood meal. These proteins are potential target for vector control strategies and development of transmission blocking vaccines.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:37:03.385.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Keshava Prasad T. S.
SpeciesList	scientific name: Anopheles stephensi (Asian malaria mosquito); NCBI TaxID: 30069;
ModificationList	iTRAQ8plex-116 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2017-05-08 01:51:20	ID requested
1	2018-01-03 02:49:37	announced
⏵ 2	2024-10-22 04:37:06	announced	2024-10-22: Updated project metadata.

Kumar M, Mohanty AK, Sreenivasamurthy SK, Dey G, Advani J, Pinto SM, Kumar A, Prasad TSK, Response to Blood Meal in the Fat Body of Anopheles stephensi Using Quantitative Proteomics: Toward New Vector Control Strategies Against Malaria. OMICS, 21(9):520-530(2017) [pubmed]

10.1089/omi.2017.0092;

curator keyword: Biological, Biomedical

submitter keyword: Juvenile hormone and Orbitrap Fusion mass spectrometer, hemolymph,Vitellogenin, egg development

T. S. Keshava Prasad
contact affiliation	Institute of Bioinformatics, International Technology Park, Bangalore, Karnataka 560066, India
contact email	keshav@ibioinformatics.org
lab head
Keshava Prasad T. S.
contact affiliation	Institute of Bioinformatics, Bangalore, Karnataka, India.
contact email	keshav@ibioinformatics.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/01/PXD006462

PRIDE project URI

• PRIDE
  1. PXD006462
     1. Label: PRIDE project
     2. Name: Identification of molecular response to blood meal in the fatbody of Anopheles stephensi using quantitative proteomics