PXD006454

PXD006454 is an original dataset announced via ProteomeXchange.

Title	Combined sperm proteome of the monarch butterfly Danaus plexippus
Description	We characterized sperm from the seminal vesicles of male monarch butterflies (Danaus plexippus), in triplicate, identifying 548 high confidence proteins. As with all but the most basal lepidopteran species male monarch butterflies are sperm heteromorphic, producing fertilization competent and anucleate fertilization incompetent sperm morphs. Comparing this data to the sperm proteomes of the Carolina sphinx moth (Manduca sexta) and the fruit fly (Drosophila melanogaster) demonstrated high levels of functional coherence across proteomes, and conservation at the level of protein abundance and post-translational modification within Lepidoptera. Comparative genomic analyses revealed a significant reduction in orthology among Monarch sperm genes relative to the remainder of the genome in non-Lepidopteran insects. A substantial number of sperm proteins were found to be specific to Lepidoptera, lacking detectable homology outside this taxa. These findings are consistent with a burst of genetic novelty in the sperm proteome concurrent with the origin of heteromorphic spermatogenesis early in Lepidoptera evolution.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:37:16.961.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Emma Whittington
SpeciesList	scientific name: Danaus plexippus; NCBI TaxID: 13037;
ModificationList	phosphorylated residue; acetylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-05-05 00:17:14	ID requested
1	2017-10-26 05:18:59	announced
2	2017-12-06 09:22:05	announced	Updated publication reference for PubMed record(s): 29197336.
⏵ 3	2024-10-22 04:37:20	announced	2024-10-22: Updated project metadata.

Whittington E, Forsythe D, Borziak K, Karr TL, Walters JR, Dorus S, Contrasting patterns of evolutionary constraint and novelty revealed by comparative sperm proteomic analysis in Lepidoptera. BMC Genomics, 18(1):931(2017) [pubmed]

10.1186/s12864-017-4293-2;

curator keyword: Biological

submitter keyword: proteomics, evolution, parasperm, sperm,monarch butterfly, lepidoptera

Dr Steve Dorus
contact affiliation	Syracuse University
contact email	sdorus@syr.edu
lab head
Emma Whittington
contact affiliation	Syracuse Univeristy
contact email	ewhittin@syr.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/10/PXD006454

PRIDE project URI

• PRIDE
  1. PXD006454
     1. Label: PRIDE project
     2. Name: Combined sperm proteome of the monarch butterfly Danaus plexippus