PXD006450

PXD006450 is an original dataset announced via ProteomeXchange.

Title	Quantitative N-terminome of N-end rule mutant in Arabidopsis
Description	According to the /N-end rule pathway, proteins with basic N-termini are targeted for degradation by the Arabidopsis thaliana E3 ligase, PROTEOLYSIS6 (PRT6). Here, we undertook a quantitative proteomics study of N-end rule mutant prt6, to investigate the impact of this pathway on the etiolated seedling. Isolation of N-terminal peptides using terminal amine isotope labelling of samples (TAILS) combined with Tandem mass tag (TMT) identified over 3000 unique N-termini. Trypsin and GluC have advantage of identification of Acetylated or neo-peptides respectively. Seed storage proteins and Cysteine proteins actives are differentially regulated in abundance in the prt6 mutants, which are represent downstream targets of transcription factors known to be N-end rule substrates.
HostingRepository	PRIDE
AnnounceDate	2017-10-27
AnnouncementXML	Submission_2017-12-04_00:26:43.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hongtao Zhang
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-05-04 05:48:51	ID requested
1	2017-10-27 06:34:06	announced
⏵ 2	2017-12-04 00:26:45	announced	Updated publication reference for PubMed record(s): 29168982.

Zhang H, Gannon L, Hassall KL, Deery MJ, Gibbs DJ, Holdsworth MJ, van der Hoorn RAL, Lilley KS, Theodoulou FL, N-terminomics reveals control of Arabidopsis seed storage proteins and proteases by the Arg/N-end rule pathway. New Phytol, 218(3):1106-1126(2018) [pubmed]

curator keyword: Biological

submitter keyword: Arabidopsis, N-terminome, N-end rule mutant etiolated seedlings TMT- labelling TAILS

Hongtao Zhang
contact affiliation	Department of Plant Sciences, Rothamsted Research, West Common, Harpenden, Herts, AL5 2JQ, UK
contact email	hongtao.zhang@rothamsted.ac.uk
lab head
Hongtao Zhang
contact affiliation	Biological Chemistry and Crop Protection Department, Rothamsted Research, Harpenden, AL5 2JQ, UK
contact email	hongtao.zhang@rothamsted.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD006450
     1. Label: PRIDE project
     2. Name: Quantitative N-terminome of N-end rule mutant in Arabidopsis