PXD006446 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomic analysis of skeletal muscle reveals estrogen as a key regulator of muscle signaling in women |
Description | Women’s aging is characterized by menopausal loss of ovarian function, which has been suggested as a contributing factor to aging-related muscle deterioration and predisposes to the metabolic dysfunctions. However, the underlying molecular mechanisms have remained unknown. To identify mechanisms, we utilized muscle samples from 24 pre- and postmenopausal women, established proteome-wide profiles and identified upstream regulators and downstream cellular pathways associated with the differences in age, menopausal status and use of hormone replacement therapy (HRT). None of the premenopausal women used hormonal medication while the postmenopausal women were monozygotic twin-sister pairs who were either current HRT users or had never used HRT. The proteomic analyses resulted in the quantification of 762 muscle proteins of which 158 were for the first time associated with female muscle aging. The Ingenuity Pathway Analysis pinpointed 17β-estradiol as a potential upstream regulator of the observed differences in the major downstream pathways including dysregulated cell death and glycolysis pathways. The results increase knowledge on the factors related to skeletal muscle signaling and aging. This is of importance, since the role of female sex hormones in the regulation of muscle cell signaling has been under appreciated and scarcely studied as compared to vast amount of data on male sex hormones and skeletal muscle. Our results clearly demonstrate the also female sex hormones and HRT should be considered as potential active players and an intervention targets to promote women’s muscular health. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:36:17.572.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Maciej Lalowski |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue |
Instrument | Synapt MS |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2017-05-03 23:34:10 | ID requested | |
1 | 2017-09-12 01:04:16 | announced | |
⏵ 2 | 2024-10-22 04:36:25 | announced | 2024-10-22: Updated project metadata. |
Publication List
Laakkonen EK, Soliymani R, Karvinen S, Kaprio J, Kujala UM, Baumann M, Sipil, ä S, Kovanen V, Lalowski M, Estrogenic regulation of skeletal muscle proteome: a study of premenopausal women and postmenopausal MZ cotwins discordant for hormonal therapy. Aging Cell, 16(6):1276-1287(2017) [pubmed] |
10.1111/acel.12661; |
Keyword List
curator keyword: Biological, Biomedical |
submitter keyword: Human, estrogen, HDMSE, aging, muscle, hormon replacement therapy |
Contact List
Marc H. Baumann |
contact affiliation | Medicum,Faculty of Medicine Biochemistry/Developmental Biology Meilahti Clinical Proteomics Core Facility PO Box 63 (Haartmaninkatu 8) FI-00014 University of Helsinki Finland |
contact email | marc.baumann@helsinki.fi |
lab head | |
Maciej Lalowski |
contact affiliation | University of Helsinki, Medicum, HiLIFE, Meilahti Clinical Proteomics Core Facility |
contact email | maciej.lalowski@helsinki.fi |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD006446
- Label: PRIDE project
- Name: Proteomic analysis of skeletal muscle reveals estrogen as a key regulator of muscle signaling in women