PXD006442

PXD006442 is an original dataset announced via ProteomeXchange.

Title	Deciphering of the human interferome and its kinetics of protein induction and repression by mass spectrometry-based quantitative proteome analysis
Description	Interferons (IFNs) are pleotropic cytokines secreted upon encounter of pathogens and tumors. Applying their antipathogenic, antiproliferative and immune stimulatory capacities, recombinant IFNs are frequently prescribed as drugs to treat different diseases. IFNs act by changing the transcription of cells. Due to characteristics like rapid induction and signaling, IFNs represent prototypic model systems for various aspects of biomedical research. In respect to the signaling and activated promoters, IFNs can be subdivided in two groups. Here, alterations of the cellular proteome of human cells treated with IFN and IFN were elucidated in a time-dependent manner by mass spectrometry-based quantitative proteome analysis. The majority of protein regulations were strongly IFN type- and time-dependent. In addition to the expected upregulation of IFN-induced proteins, an astonishing number of proteins become profoundly repressed especially by IFN. Taken together, our comprehensive analysis deciphers the human interferome and its kinetics of protein induction and repression.
HostingRepository	PRIDE
AnnounceDate	2018-05-25
AnnouncementXML	Submission_2018-05-25_02:48:11.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD006442
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Dominik Andre Meger
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Oxidation; Carbamidomethyl
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2017-05-03 07:13:37	ID requested
⏵ 1	2018-05-25 02:48:12	announced

Megger DA, Philipp J, Le-Trilling VTK, Sitek B, Trilling M, Deciphering of the Human Interferon-Regulated Proteome by Mass Spectrometry-Based Quantitative Analysis Reveals Extent and Dynamics of Protein Induction and Repression. Front Immunol, 8():1139(2017) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: Label-free quantification, interferome, interferon alpha, interferon gamma

Dominik Andre Megger
contact affiliation	1) Medizinisches Proteom-Center, Ruhr-Universität Bochum, Universitätsstraße 150, 44801 Bochum, Germany 2) Institute of Virology, University Hospital, University Duisburg-Essen, Hufelandstr. 55, 45120 Essen, Germany
contact email	dominik.megger@rub.de
lab head
Dominik Andre Meger
contact affiliation	Medizinisches Proteom-Center (MPC), Ruhr-Universität Bochum, Germany
contact email	dominik.megger@rub.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/05/PXD006442

PRIDE project URI

• PRIDE
  1. PXD006442
     1. Label: PRIDE project
     2. Name: Deciphering of the human interferome and its kinetics of protein induction and repression by mass spectrometry-based quantitative proteome analysis