PXD006441

PXD006441 is an original dataset announced via ProteomeXchange.

Title	Multipronged quantitative proteomics reveals serum proteome alterations in breast cancer intrinsic subtypes
Description	Being molecularly heterogeneous, breast cancer tends to be a complicated oncological disease with high incidence rates throughout the world. The primary aim of this study was to identify the set of serum proteins with discriminatory capabilities towards the four major subtypes of breast cancer. We employed multipronged quantitative proteomic approaches like 2D-DIGE, iTRAQ and SWATH-MS and identified 307 differentially regulated proteins. Luminal A subtype consisted of 24, Luminal B subtype 38, HER2 Enriched subtype 17 and Triple negative breast cancer subtype 10 differentially regulated subtype specific proteins. These specific proteins were further subjected to bioinformatic analysis viz. PANTHER, DAVID and LENS which revealed the involvement in platelet degranulation, fibrinolysis, lipid metabolism, immune response, complement activation, blood coagulation, immune cell activation, glycolysis, amino acid biosynthesis and cancer signaling pathways in the subtypes of the breast cancer. The significant discrimination efficiency of the models generated through multivariate statistical analysis was decent to distinguish each of the four subtypes from controls. Further, some of the statistically significant differentially regulated proteins were verified and validated by immunoblotting and mass spectrometry based selected reaction monitoring (SRM) approach. Our Multipronged proteomics approaches revealed panel of serum proteins specifically altered for individual subtypes of breast cancer.
HostingRepository	PRIDE
AnnounceDate	2017-05-15
AnnouncementXML	Submission_2017-05-15_01:02:41.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Srikanth Rapole
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2017-05-03 05:33:51	ID requested
⏵ 1	2017-05-15 01:02:42	announced

Gajbhiye A, Dabhi R, Taunk K, Jagadeeshaprasad MG, RoyChoudhury S, Mane A, Bayatigeri S, Chaudhury K, Santra MK, Rapole S, Multipronged quantitative proteomics reveals serum proteome alterations in breast cancer intrinsic subtypes. J Proteomics, 163():1-13(2017) [pubmed]

curator keyword: Biomedical

submitter keyword: Breast Cancer, DIGE, iTRAQ, SWATH, Serum Biomarkers, Proteomics

SRIKANTH RAPOLE
contact affiliation	Proteomics Lab, National Centre for Cell Science, Pune, Maharashtra, India.
contact email	rsrikanth@nccs.res.in
lab head
Srikanth Rapole
contact affiliation	NCCS, Pune
contact email	nccsproteomics@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD006441
     1. Label: PRIDE project
     2. Name: Multipronged quantitative proteomics reveals serum proteome alterations in breast cancer intrinsic subtypes