PXD006436

PXD006436 is an original dataset announced via ProteomeXchange.

Title	Exhaled breath condensate biomarkers for the early diagnosis of lung cancer using proteomics
Description	The present study explored whether the proteomic analysis of exhaled breath condensate (EBC) may provide potential biomarkers for their use in non-invasive screening strategies for the early detection of lung cancer (LC). EBC was collected from 192 individuals (49 control volunteers, 49 risk factor-smoking volunteers, 46 chronic obstructive pulmonary disease patients and 48 LC patients). Using LC-MS/MS a total of 348 different proteins with a different pattern among the four groups were identified in EBC samples. Despite the great variability observed among individuals, significantly more proteins were identified in the EBC from LC patients compared to other groups. Furthermore, the average number of proteins identified per sample was significantly higher in LC patients and Receiver Operating Characteristic curve analysis showed an area under the curve of 0.8, indicating diagnostic value. Proteins frequently detected in EBC, such as dermcidin and hornerin, along with others much less frequently detected, such as hemoglobin and histone isoforms, were identified. Cytokeratins (KRTs) were the most abundant proteins in EBC samples and levels of KRT6A, KRT6B and KRT6C isoforms were significantly higher in samples from LC patients. Moreover, the amount of most KRTs in EBC samples from LC patients showed a significant positive correlation with tumor size. Finally, we used a random forest algorithm to generate a robust model using EBC protein data for the diagnosis of patients with LC. Thus, this study demonstrates that the proteomic analysis of EBC samples is an appropriated approach to develop biomarkers for the diagnosis of lung cancer.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:37:38.934.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD006436
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Luz Valero
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Deamidated; Oxidation; Carbamidomethyl
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2017-05-03 02:46:20	ID requested
1	2019-08-16 01:23:27	announced
⏵ 2	2024-10-22 04:37:39	announced	2024-10-22: Updated project metadata.

L, ó, pez-S, á, nchez LM, Jurado-G, á, mez B, Feu-Collado N, Valverde A, Ca, ñ, as A, Fern, á, ndez-Rueda JL, Aranda E, Rodr, í, guez-Ariza A, Exhaled breath condensate biomarkers for the early diagnosis of lung cancer using proteomics. Am J Physiol Lung Cell Mol Physiol, 313(4):L664-L676(2017) [pubmed]

10.6019/PXD006436;

10.1152/ajplung.00119.2017;

curator keyword: Biomedical

submitter keyword: proteomics,exhaled breath condensate, biomarkers, lung cancer

Antonio Rodríguez-Ariza
contact affiliation	Instituto Maimónides de Investigación Biomédica de Córdoba, Córdoba, Spain. Centro de Investigación Biomédica en Red en Cáncer (CIBER-ONC), Madrid, Spain Unidad de Gestión Clínica de Oncología Médica, Hospital Universitario Reina Sofía, Spain.
contact email	antonio.rodriguez.exts@juntadeandalucia.es
lab head
Luz Valero
contact affiliation	Universitat de Valencia
contact email	mluz.valero@uv.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/08/PXD006436

PRIDE project URI

• PRIDE
  1. PXD006436
     1. Label: PRIDE project
     2. Name: Exhaled breath condensate biomarkers for the early diagnosis of lung cancer using proteomics