PXD006425

PXD006425 is an original dataset announced via ProteomeXchange.

Title	In depth proteome profiling of Cumin by LTQ-Orbitrap Velos mass spectrometer
Description	A shotgun proteomic approach was used to obtain comprehensive dataset of cumin proteins. For this purpose we applied a one dimensional electrophoresis coupled with high resolution mass spectrometry. The first step in our proteome analysis strategy, we evaluate different extraction methods, based on differential solubility of protein. Among those methods, Readyprep protein extraction kit provide high purity of protein extract, gave higher protein yield and typically good resolution of protein bands on one dimensional gel electrophoresis. Further nanoLC-MS/MS analysis by state-of-the-art linear ion trap (LTQ)-Orbitrap Velose mass spectrometer resulted in identification of very high number proteins. Bioinformatics analysis provide functional distribution of identified protein. To our knowledge, this is the first study aimed for in depth study of cumin proteome.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:48:44.146.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Uzma Rana
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2017-05-02 05:44:12	ID requested
1	2022-02-28 02:56:10	announced
⏵ 2	2023-11-14 08:48:44	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

curator keyword: Biological

submitter keyword: Plant proteins, Cumin, LC-MS/MS

Uzma Zaman
contact affiliation	Dow Research Institute of Biotechnology and Biomedical Sciences, Dow University of Health Sciences
contact email	uzma.zaman@duhs.edu.pk
lab head
Uzma Rana
contact affiliation	Dow University of Health Sciences
contact email	uzma.zaman@duhs.edu.pk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD006425

PRIDE project URI

• PRIDE
  1. PXD006425
     1. Label: PRIDE project
     2. Name: In depth proteome profiling of Cumin by LTQ-Orbitrap Velos mass spectrometer