PXD006421

PXD006421 is an original dataset announced via ProteomeXchange.

Title	Shotgun mass-spectrometry of HL-60 cell proteins for spectrum library development
Description	ATRA-induced differentiation of HL-60 cells was studied using targeted mass-spectrometry including selected reaction monitoring (SRM) and parallel reaction monitoring (PRM) approach. PRM experiment was performed in time-course manner, without peptide standards usage. PRM data was inspected in Skyline 3.1 software. In order to check peptide identity we developed spectrum library based on shotgun mass-spectrometry data, which has been obtained for HL-60 cells protein samples at 0, 3, 24, 48 and 96h after ATRA treatment.
HostingRepository	PRIDE
AnnounceDate	2017-11-09
AnnouncementXML	Submission_2017-11-09_06:34:56.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD006421
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Svetlana Novikova
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Oxidation; Carbamidomethyl
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2017-05-02 04:49:20	ID requested
⏵ 1	2017-11-09 06:34:57	announced

Novikova SE, Tikhonova OV, Kurbatov LK, Farafonova TE, Vakhrushev IV, Zgoda VG, Application of selected reaction monitoring and parallel reaction monitoring for investigation of HL-60 cell line differentiation. Eur J Mass Spectrom (Chichester), 23(4):202-208(2017) [pubmed]

curator keyword: Technical, Biomedical

submitter keyword: HL-60 cell line, Orbitrap Velos, ATRA, differentiation

Victor Zgoda
contact affiliation	Laboratory of systems biology, IBMC, Moscow, Russia
contact email	victor.zgoda@gmail.com
lab head
Svetlana Novikova
contact affiliation	institute of biomedical chemistry Moscow
contact email	novikova.s.e3101@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD006421

PRIDE project URI

• PRIDE
  1. PXD006421
     1. Label: PRIDE project
     2. Name: Shotgun mass-spectrometry of HL-60 cell proteins for spectrum library development