PXD006419

PXD006419 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteomic characterization of lung-MSC and bone marrow-MSC using DIA-mass spectrometry
Description	Mesenchymal stromal cells (MSC) are ideal candidates for cell therapies, due to their immune-regulatory and regenerative properties. We have previously reported that lung-derived MSC are tissue-resident cells with lung-specific properties compared to bone marrow-derived MSC. Assessing relevant molecular differences between lung-MSC and bone marrow-MSC is important, given that such differences may impact their behavior and potential therapeutic use. Here, we present an in-depth mass spectrometry (MS) based strategy to investigate the proteomes of lung-MSC and bone marrow-MSC. The MS-strategy relies on label free quantitative data-independent acquisition (DIA) analysis and targeted data analysis using a MSC specific spectral library. We identified several significantly differentially expressed proteins between lung-MSC and bone marrow-MSC within the cell layer (352 proteins) and in the conditioned medium (49 proteins). Bioinformatics analysis revealed differences in regulation of cell proliferation, which was functionally confirmed by decreasing proliferation rate through Cytochrome P450 stimulation. Our study reveals important tissue-specific differences within proteome and matrisome profiles between lung- and bone marrow-derived MSC that may influence their behavior and affect the clinical outcome when used for cell-therapy.
HostingRepository	PRIDE
AnnounceDate	2018-10-18
AnnouncementXML	Submission_2018-10-18_11:48:41.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sara Rolandsson Enes
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-05-02 01:52:00	ID requested
⏵ 1	2018-10-18 11:48:45	announced

Rolandsson Enes S, Å, hrman E, Palani A, Hallgren O, Bjermer L, Malmstr, ö, m A, Scheding S, Malmstr, ö, m J, Westergren-Thorsson G, Quantitative proteomic characterization of lung-MSC and bone marrow-MSC using DIA-mass spectrometry. Sci Rep, 7(1):9316(2017) [pubmed]

curator keyword: Biomedical

submitter keyword: Human

Gunilla Westergren-Thorsson
contact affiliation	Department of Experimental Medical Science, Lung Biology Unit, Lund University, 22184, Lund, Sweden
contact email	gunilla.westergren-thorsson_enes@med.lu.se
lab head
Sara Rolandsson Enes
contact affiliation	Lund University
contact email	sara.rolandsson_enes@med.lu.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD006419

PRIDE project URI

• PRIDE
  1. PXD006419
     1. Label: PRIDE project
     2. Name: Quantitative proteomic characterization of lung-MSC and bone marrow-MSC using DIA-mass spectrometry