PXD006412

PXD006412 is an original dataset announced via ProteomeXchange.

Title	Gastrointestinal digestion of hazelnut allergens on molecular level: Elucidation of degradation kinetics and resistant immunoactive peptides using mass spectrometry
Description	Allergy to hazelnut seeds ranks among the most prevalent food allergies in Europe. The aim of this study was to elucidate the gastrointestinal digestion of hazelnut allergens on molecular level. Hazelnut flour was digested in vitro following the Infogest consensus model. For six allergenic proteins, the time-dependent course of digestion was monitored by SDS-PAGE and HPLC−MS/MS, and degradation products were characterized by a bottom-up proteomics approach. Depending on the molecular structure, a specific biochemical fate was observed for each allergen, and degradation kinetics were traced back to the peptide level. 1183 peptides were characterized, including 130 peptides that carry known IgE-binding epitopes and may represent sensitizers for hazelnut allergy. The kinetics of peptide formation and degradation were determined by label free quantification and follow a complex multi-stage mechanism.
HostingRepository	PRIDE
AnnounceDate	2017-07-11
AnnouncementXML	Submission_2017-07-11_04:33:11.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Robin Korte
SpeciesList	scientific name: Corylus avellana; NCBI TaxID: 13451;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap XL

Revision	Datetime	Status	ChangeLog Entry
0	2017-05-02 00:32:19	ID requested
⏵ 1	2017-07-11 04:33:12	announced

Korte R, Br, ä, cker J, Brockmeyer J, Gastrointestinal digestion of hazelnut allergens on molecular level: Elucidation of degradation kinetics and resistant immunoactive peptides using mass spectrometry. Mol Nutr Food Res, 61(10):(2017) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: Allergen, Digestion, Food allergy, Hazelnut

Jens Brockmeyer
contact affiliation	Analytical Food Chemistry, University of Stuttgart, Germany
contact email	jens.brockmeyer@lc.uni-stuttgart.de
lab head
Robin Korte
contact affiliation	University of Münster, Germany
contact email	robin.korte@uni-muenster.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/07/PXD006412

PRIDE project URI

• PRIDE
  1. PXD006412
     1. Label: PRIDE project
     2. Name: Gastrointestinal digestion of hazelnut allergens on molecular level: Elucidation of degradation kinetics and resistant immunoactive peptides using mass spectrometry