PXD006410

PXD006410 is an original dataset announced via ProteomeXchange.

Title	Affinity chromatography-based identification of sRNA protein partners in Rhizobia
Description	This project aims to identify novel protein partners of conserved trans-sRNAs in the α-proteobacterium Sinorhizobium meliloti. Trans-sRNAs (AbcR2, NfeR1 and EcpR1) were tagged at their 5’ ends with the MS2 aptamer. To identify the proteins associated with the trans-sRNAs we: i) expressed and purified the MS2 coat protein fused to maltose-binding protein (MS2-MBP); ii) purified MS2 tagged sRNAs conjugated with MS2-MBP via amylase column; and iii) subjected retained proteins to mass spectrometry analysis. To discard unspecific interactions we also analysed several control samples: i) samples expressing untagged trans-sRNAs; ii) samples expressing an MS2 tagged Rho-independent transcriptional terminator.
HostingRepository	PRIDE
AnnounceDate	2020-10-19
AnnouncementXML	Submission_2020-10-19_01:14:31.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ana Matia Gonzalez
SpeciesList	scientific name: Rhizobium meliloti (Ensifer meliloti) (Sinorhizobium meliloti); NCBI TaxID: 382;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	amaZon Speed ETD

Revision	Datetime	Status	ChangeLog Entry
0	2017-04-28 08:01:39	ID requested
⏵ 1	2020-10-19 01:14:32	announced

Robledo M, Garc, í, a-Tomsig NI, Matia-Gonz, á, lez AM, Garc, í, a-Rodr, í, guez FM, Jim, é, nez-Zurdo JI, -rhizobia can bind functionally diverse RNA species. RNA Biol, 18(8):1111-1123(2021) [pubmed]

curator keyword: Biological

submitter keyword: Sinorhizobium meliloti, trans-sRNAs , MS2 aptamer, LC-MS/MS

José Ignacio Jiménez Zurdo
contact affiliation	Dpt. Microbiología del Suelo y Sistemas Simbióticos Estación Experimental del Zaidín, CSIC Profesor Albareda, 1 18008 Granada (Spain) Tlf. +34 958181600 Ext. 176
contact email	joseignacio.jimenez@eez.csic.es
lab head
Ana Matia Gonzalez
contact affiliation	University of Surrey
contact email	a.matia@surrey.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/10/PXD006410

PRIDE project URI

• PRIDE
  1. PXD006410
     1. Label: PRIDE project
     2. Name: Affinity chromatography-based identification of sRNA protein partners in Rhizobia