PXD006398

PXD006398 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Profiling Endogenous SUMO and Ub Modifications Side-by-Side under Native Conditions
Description	Datasets of the proteome-wide profiling of native SUMO post-translational modifications in HCT and HELA cells using a novel method to isolate modified peptides through a diglycyl lysine remnant, as previously used for proteome-wide ubiquitin profiling. Includes ID experiments for the identification of SUMO modification sites, quantification of the effects of proteome inhibition by MG132 using K8 SILAC methods, and side-by-side identification of ubiquitin and sumoylation modification sites.
HostingRepository	MassIVE
AnnounceDate	2017-08-23
AnnouncementXML	Submission_2017-08-23_10:44:13.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Ryan Lumpkin
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090;
ModificationList	Label:13C(6)15N(2); Label:13C(6)15N(2)+GG; GG; Nethylmaleimide; Oxidation; Acetyl; Gln->pyro-Glu
Instrument	LTQ Orbitrap Velos; LTQ Orbitrap Elite; Orbitrap Fusion; Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2017-04-27 09:04:41	ID requested
⏵ 1	2017-08-23 10:44:14	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: SUMO, SUMO remnant, Ubiquitin, KGG, digly, SUMO profiling, MG132, wAlp, wild type alphalytic protease

submitter keyword: SUMO, SUMO remnant, Ubiquitin, KGG, digly, SUMO profiling, MG132, wAlp, wild type alphalytic protease

Contact List

Elizabeth Komives
contact affiliation	University of California, San Diego
contact email	ekomives@ucsd.edu
lab head
Ryan Lumpkin
contact affiliation	UCSD
contact email	rlumpkin@ucsd.edu
dataset submitter

Full Dataset Link List

MassIVE dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000081018

MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000081018