PXD006395

PXD006395 is an original dataset announced via ProteomeXchange.

Title	Global Analyses of Selective Insulin Resitance in Hepatocytes due to Palmitate Lipotoxicity
Description	Obesity is tightly linked to hepatic steatosis and insulin resistance. One feature of this association is the paradox of selective insulin resistance: insulin fails to suppress hepatic gluconeogenesis but activates lipid synthesis in the liver. How lipid accumulation interferes selectively with some branches of hepatic insulin signaling is not well understood. Here we provide a resource, based on unbiased approaches and established in a simple cell culture system, to enable investigations of the phenomenon of selective insulin resistance. We analyzed the phosphoproteome of insulin-treated human hepatoma cells and identified sites in which palmitate selectively impairs insulin signaling. As an example, we show that palmitate interferes with insulin signaling to FoxO1, a key transcription factor regulating gluconeogenesis, and identify a possible mechanism. This model system, together with our comprehensive characterization of the proteome, phosphoproteome, and lipidome changes in response to palmitate treatment, provides a novel and useful resource for unraveling the mechanisms underlying selective insulin resistance.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:42:24.195.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zon Weng Lai
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-04-27 01:32:48	ID requested
1	2018-02-14 04:11:29	announced
⏵ 2	2024-10-22 04:42:32	announced	2024-10-22: Updated project metadata.

10.1074/mcp.ra117.000560;

Li Z, Lai ZW, Christiano R, Gazos-Lopes F, Walther TC, Farese RV, Global Analyses of Selective Insulin Resistance in Hepatocytes Caused by Palmitate Lipotoxicity. Mol Cell Proteomics, 17(5):836-849(2018) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: insulin resistance, lipotoxicity, phosphoproteomics,hepatic steatosis

Tobias Walther
contact affiliation	Harvard School of Public Health
contact email	twalther@hsph.harvard.edu
lab head
Zon Weng Lai
contact affiliation	Harvard T. H. Chan School of Public Health
contact email	zlai@hsph.harvard.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/02/PXD006395

PRIDE project URI

• PRIDE
  1. PXD006395
     1. Label: PRIDE project
     2. Name: Global Analyses of Selective Insulin Resitance in Hepatocytes due to Palmitate Lipotoxicity