PXD006395 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Global Analyses of Selective Insulin Resitance in Hepatocytes due to Palmitate Lipotoxicity |
Description | Obesity is tightly linked to hepatic steatosis and insulin resistance. One feature of this association is the paradox of selective insulin resistance: insulin fails to suppress hepatic gluconeogenesis but activates lipid synthesis in the liver. How lipid accumulation interferes selectively with some branches of hepatic insulin signaling is not well understood. Here we provide a resource, based on unbiased approaches and established in a simple cell culture system, to enable investigations of the phenomenon of selective insulin resistance. We analyzed the phosphoproteome of insulin-treated human hepatoma cells and identified sites in which palmitate selectively impairs insulin signaling. As an example, we show that palmitate interferes with insulin signaling to FoxO1, a key transcription factor regulating gluconeogenesis, and identify a possible mechanism. This model system, together with our comprehensive characterization of the proteome, phosphoproteome, and lipidome changes in response to palmitate treatment, provides a novel and useful resource for unraveling the mechanisms underlying selective insulin resistance. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:42:24.195.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Zon Weng Lai |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2017-04-27 01:32:48 | ID requested | |
1 | 2018-02-14 04:11:29 | announced | |
⏵ 2 | 2024-10-22 04:42:32 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1074/mcp.ra117.000560; |
Li Z, Lai ZW, Christiano R, Gazos-Lopes F, Walther TC, Farese RV, Global Analyses of Selective Insulin Resistance in Hepatocytes Caused by Palmitate Lipotoxicity. Mol Cell Proteomics, 17(5):836-849(2018) [pubmed] |
Keyword List
curator keyword: Biological, Biomedical |
submitter keyword: insulin resistance, lipotoxicity, phosphoproteomics,hepatic steatosis |
Contact List
Tobias Walther |
contact affiliation | Harvard School of Public Health |
contact email | twalther@hsph.harvard.edu |
lab head | |
Zon Weng Lai |
contact affiliation | Harvard T. H. Chan School of Public Health |
contact email | zlai@hsph.harvard.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD006395
- Label: PRIDE project
- Name: Global Analyses of Selective Insulin Resitance in Hepatocytes due to Palmitate Lipotoxicity