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PXD006395

PXD006395 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleGlobal Analyses of Selective Insulin Resitance in Hepatocytes due to Palmitate Lipotoxicity
DescriptionObesity is tightly linked to hepatic steatosis and insulin resistance. One feature of this association is the paradox of selective insulin resistance: insulin fails to suppress hepatic gluconeogenesis but activates lipid synthesis in the liver. How lipid accumulation interferes selectively with some branches of hepatic insulin signaling is not well understood. Here we provide a resource, based on unbiased approaches and established in a simple cell culture system, to enable investigations of the phenomenon of selective insulin resistance. We analyzed the phosphoproteome of insulin-treated human hepatoma cells and identified sites in which palmitate selectively impairs insulin signaling. As an example, we show that palmitate interferes with insulin signaling to FoxO1, a key transcription factor regulating gluconeogenesis, and identify a possible mechanism. This model system, together with our comprehensive characterization of the proteome, phosphoproteome, and lipidome changes in response to palmitate treatment, provides a novel and useful resource for unraveling the mechanisms underlying selective insulin resistance.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:42:24.195.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterZon Weng Lai
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListiodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02017-04-27 01:32:48ID requested
12018-02-14 04:11:29announced
22024-10-22 04:42:32announced2024-10-22: Updated project metadata.
Publication List
10.1074/mcp.ra117.000560;
Li Z, Lai ZW, Christiano R, Gazos-Lopes F, Walther TC, Farese RV, Global Analyses of Selective Insulin Resistance in Hepatocytes Caused by Palmitate Lipotoxicity. Mol Cell Proteomics, 17(5):836-849(2018) [pubmed]
Keyword List
curator keyword: Biological, Biomedical
submitter keyword: insulin resistance, lipotoxicity, phosphoproteomics,hepatic steatosis
Contact List
Tobias Walther
contact affiliationHarvard School of Public Health
contact emailtwalther@hsph.harvard.edu
lab head
Zon Weng Lai
contact affiliationHarvard T. H. Chan School of Public Health
contact emailzlai@hsph.harvard.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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