PXD006393

PXD006393 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis to investigate lung injury in a mouse model of Streptococcus pneumonia infection
Description	Infections by Streptococcus pneumoniae are a major cause of morbidity and mortality worldwide, often causing community-acquired pneumonia, otitis media and also bacteremia and meningitis. Studies on S. pneumoniae are mainly focused on its virulence or capability to evade the host immune system, but little is known about the injury caused in lungs during a pneumococcal infection. Herein we compared the proteome profile of lungs from S. pneumoniae-infected mice with control mice by means of DIGE technology. In order to obtain reliable results three biological replicas were used, and four technical replicas were carried out for each biological replica. Proteomic comparison was performed at two time points: 24 and 48 hours post infection. A total of 91 proteins were identified with different abundance. We found important changes in the protein profiles during pneumococcal infection mainly associated with regulation of vesicle-mediated transport, wound healing, and cytoskeleton organization. In conclusion, S. pneumoniae may manipulate cytoskeleton of the cell during a lung infection likely by the death of eukaryotic cells.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:36:59.151.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD006393
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	MARIO Ferrer-Navarro
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	ultraflex

Revision	Datetime	Status	ChangeLog Entry
0	2017-04-26 06:38:28	ID requested
1	2018-06-10 14:34:30	announced
⏵ 2	2024-10-22 04:37:02	announced	2024-10-22: Updated project metadata.

10.6019/PXD006393;

Ferrer-Navarro M, Strehlitz A, Medina E, Vila J, Infection. Front Microbiol, 9():928(2018) [pubmed]

10.3389/fmicb.2018.00928;

curator keyword: Biological, Biomedical

submitter keyword: lung injury, DIGE, pneumonia,Streptococcus pneumoniae

Jordi Vila
contact affiliation	Professor of Microbiology Hospital Clinic Head of the Department of Clinical Microbiology School of Medicine, University of Barcelona Barcelona, Spain
contact email	JVILA@clinic.cat
lab head
MARIO Ferrer-Navarro
contact affiliation	ISGlobal
contact email	mario.ferrer@isglobal.org
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD006393
     1. Label: PRIDE project
     2. Name: Proteomic analysis to investigate lung injury in a mouse model of Streptococcus pneumonia infection