PXD006389

PXD006389 is an original dataset announced via ProteomeXchange.

Title	The Ser/Thr Protein Kinase Protein-Protein Interaction Map of M. tuberculosis
Description	Mycobacterium tuberculosis (Mtb) is the causative agent of tuberculosis, the leading cause of death among all infectious diseases. There are 11 eukaryotic-like serine/threonine protein kinases (STPKs) in Mtb, which are thought to play pivotal roles in cell growth, signal transduction and pathogenesis. However, their underlying mechanisms of action remain largely uncharacterized. In this study, using a Mtb proteome microarray, we have globally identified the binding proteins in Mtb for all of the STPKs, and constructed the first STPK protein interaction (KPI) map that includes 492 binding proteins and 1,027 interactions. Bioinformatics analysis showed that the interacting proteins reflect diverse functions, including roles in two-component system, transcription, protein degradation, and cell wall integrity. Functional investigations confirmed that PknG regulates cell wall integrity through key components of peptidoglycan (PG) biosynthesis, e.g., MurC. The global STPK-KPIs network constructed here is expected to serve as a rich resource for understanding the key signaling pathways in Mtb, thus facilitating drug development and effective control of Mtb.
HostingRepository	PRIDE
AnnounceDate	2017-06-06
AnnouncementXML	Submission_2017-06-06_03:03:36.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD006389
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Fanlin Wu
SpeciesList	scientific name: Mycobacterium tuberculosis H37Rv; NCBI TaxID: 83332;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-04-26 04:17:45	ID requested
⏵ 1	2017-06-06 03:03:37	announced

Wu FL, Liu Y, Jiang HW, Luan YZ, Zhang HN, He X, Xu ZW, Hou JL, Ji LY, Xie Z, Czajkowsky DM, Yan W, Deng JY, Bi LJ, Zhang XE, Tao SC, . Mol Cell Proteomics, 16(8):1491-1506(2017) [pubmed]

ProteomeXchange project tag: Human Proteome Project

curator keyword: Biomedical

submitter keyword: Ser/Thr protein kinase, Protein-protein interaction, Cell wall, MurC

Sheng-Ce Tao
contact affiliation	Ph.D., Professor, Vice Dean
contact email	taosc@sjtu.edu.cn
lab head
Fanlin Wu
contact affiliation	Shanghai Jiao Tong University
contact email	wufanlin1990@126.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/06/PXD006389

PRIDE project URI

• PRIDE
  1. PXD006389
     1. Label: PRIDE project
     2. Name: The Ser/Thr Protein Kinase Protein-Protein Interaction Map of M. tuberculosis