PXD006384

PXD006384 is an original dataset announced via ProteomeXchange.

Title	Metaproteomics of colonic microbiota unveils discrete protein functions among colitic mice and control groups
Description	We utilized the well-characterized murine T cell transfer model of colitis to find specific alterations in the intestinal luminal proteome associated with inflammation. Mass spectrometry proteomic analysis of colonic samples permitted the identification of ~10,000-12,000 unique peptides that corresponded to 5610 protein clusters identified across three groups, including the colitic Rag1 -/- T cell recipients, isogenic Rag1 -/- controls, as well as wild-type mice. Bioinformatic analyses on host and microbial proteins found specific proteins and GO term functionalities unique to each group, as well as GO terms shared across the three cohorts. We further demonstrated that the colitic mice exhibited a significant increase in Proteobacteria and Verrucomicrobia that was substantiated with 16S rDNA sequencing.
HostingRepository	PRIDE
AnnounceDate	2018-07-05
AnnouncementXML	Submission_2018-07-05_05:15:24.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Greg Stupp
SpeciesList	scientific name: human gut metagenome; NCBI TaxID: 408170;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap XL

Revision	Datetime	Status	ChangeLog Entry
0	2017-04-26 01:50:58	ID requested
⏵ 1	2018-07-05 05:15:25	announced

Moon C, Stupp GS, Su AI, Wolan DW, Metaproteomics of Colonic Microbiota Unveils Discrete Protein Functions among Colitic Mice and Control Groups. Proteomics, 18(3-4):(2018) [pubmed]

curator keyword: Metaproteomics, Biological, Biomedical

submitter keyword: Metaproteomics, MudPIT, ComPIL, Microbiota, Inflammatory Bowel Disease, murine colitis, Rag1 -/- , T cell transfer, 16S sequencing

Dennis Wolan
contact affiliation	Assistant Professor Department of Molecular and Experimental Medicine TSRI - California Campus USA
contact email	wolan@scripps.edu
lab head
Greg Stupp
contact affiliation	TSRI
contact email	gstupp@scripps.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/07/PXD006384

PRIDE project URI

• PRIDE
  1. PXD006384
     1. Label: PRIDE project
     2. Name: Metaproteomics of colonic microbiota unveils discrete protein functions among colitic mice and control groups